PMID- 17897629
OWN - NLM
STAT- MEDLINE
DCOM- 20080211
LR  - 20161124
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 363
IP  - 3
DP  - 2007 Nov 23
TI  - Function of oxidative stress in the regulation of hematopoietic stem cell-niche 
      interaction.
PG  - 578-83
AB  - During postnatal life, the bone marrow (BM) supports both self-renewal and 
      differentiation of hematopoietic stem cells (HSCs) in specialized niches, such as 
      osteoblastic niche and vascular niche. A cell adhesion molecule, N-cadherin 
      expressed in the HSCs and osteoblasts, suggesting that homophylic binding of 
      N-cadherin induce the adhesion of HSCs to the niche cells. Here we demonstrate 
      that an anti-cancer drug, 5-fuluorouracil induces reactive oxygen species (ROS) 
      in HSCs, which suppressed N-cadherin expression. These events result in the shift 
      of side population (SP) cells to non-SP cells, indicating that quiescent HSCs are 
      detached from the niche. Administration of a potent anti-oxidant, N-acetyl 
      cystein (NAC) suppressed the shift from SP cells. These data suggest that ROS 
      suppressed the N-cadherin-mediated cell adhesion, and induce the exit of HSCs 
      from the niche.
FAU - Hosokawa, Kentaro
AU  - Hosokawa K
AD  - Department of Cell Differentiation, The Sakaguchi Laboratory of Developmental 
      Biology, School of Medicine, Keio University, 35 Shinano-machi, Shinjuku-ku, 
      Tokyo 160-8582, Japan.
FAU - Arai, Fumio
AU  - Arai F
FAU - Yoshihara, Hiroki
AU  - Yoshihara H
FAU - Nakamura, Yuka
AU  - Nakamura Y
FAU - Gomei, Yumiko
AU  - Gomei Y
FAU - Iwasaki, Hiroko
AU  - Iwasaki H
FAU - Miyamoto, Kana
AU  - Miyamoto K
FAU - Shima, Haruko
AU  - Shima H
FAU - Ito, Keisuke
AU  - Ito K
FAU - Suda, Toshio
AU  - Suda T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070918
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Cadherins)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Free Radical Scavengers)
RN  - 0 (Imidazoles)
RN  - 0 (Pyridines)
RN  - 0 (RNA, Messenger)
RN  - 0 (Reactive Oxygen Species)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
RN  - OU13V1EYWQ (SB 203580)
RN  - U3P01618RT (Fluorouracil)
RN  - WYQ7N0BPYC (Acetylcysteine)
SB  - IM
MH  - Acetylcysteine/pharmacology
MH  - Animals
MH  - Bone Marrow Cells/cytology/drug effects/metabolism
MH  - Cadherins/genetics/metabolism
MH  - Cell Count
MH  - Cells, Cultured
MH  - Enzyme Inhibitors/pharmacology
MH  - Flow Cytometry
MH  - Fluorouracil/pharmacology
MH  - Free Radical Scavengers/pharmacology
MH  - Gene Expression/drug effects
MH  - Hematopoietic Stem Cells/cytology/drug effects/*metabolism
MH  - Imidazoles/pharmacology
MH  - Immunohistochemistry
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Oxidative Stress/*physiology
MH  - Pyridines/pharmacology
MH  - RNA, Messenger/genetics/metabolism
MH  - Reactive Oxygen Species/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Time Factors
MH  - p38 Mitogen-Activated Protein Kinases/antagonists & 
      inhibitors/genetics/metabolism
EDAT- 2007/09/28 09:00
MHDA- 2008/02/12 09:00
CRDT- 2007/09/28 09:00
PHST- 2007/08/29 00:00 [received]
PHST- 2007/09/05 00:00 [accepted]
PHST- 2007/09/28 09:00 [pubmed]
PHST- 2008/02/12 09:00 [medline]
PHST- 2007/09/28 09:00 [entrez]
AID - S0006-291X(07)01955-9 [pii]
AID - 10.1016/j.bbrc.2007.09.014 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2007 Nov 23;363(3):578-83. doi: 
      10.1016/j.bbrc.2007.09.014. Epub 2007 Sep 18.