PMID- 17897817
OWN - NLM
STAT- MEDLINE
DCOM- 20080530
LR  - 20220316
IS  - 0944-7113 (Print)
IS  - 0944-7113 (Linking)
VI  - 15
IP  - 1-2
DP  - 2008 Jan
TI  - Apoptotic effect of hesperidin through caspase3 activation in human colon cancer 
      cells, SNU-C4.
PG  - 147-51
AB  - Hesperidin, a known flavonoid constituent of citrus, reduces the proliferation of 
      many cancer cells. The apoptotic effects of hesperidin on human colon cancer 
      cells, SNU-C4, were determined at concentrations of 1-100 microM. At 100 microM, 
      hesperidin reduced cell viability to 65.00+/-0.05% of control values in a 
      3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Cell 
      death induced by hesperidin showed apoptotic features in 
      4,6-diamidino-2-phenylindole (DAPI) staining and in terminal deoxynucleotidyl 
      transferase-mediated dUTP nick end labeling (TUNEL) assays. Examination of the 
      expression of apoptosis-regulating genes indicated that hesperidin treatment 
      decreased the expression of B-cell CLL/lymphoma 2 (BCL2) mRNA, and increased the 
      expression of BCL2-associated X protein (BAX). The expression and activity of the 
      major apoptotic factor caspase3 (CASP3) was increased significantly with 
      hesperidin treatment. Hesperidin down-regulated the protein expression of 
      pro-CASP3, and up-regulated the level of active CASP3. Thus, these results 
      suggest that hesperidin could induce apoptosis in human colon cancer cells 
      through CASP3 activation.
FAU - Park, H J
AU  - Park HJ
AD  - Kohwang Medical Research Institute, College of Medicine, Kyung Hee University, 
      Seoul 130-701, Republic of Korea.
FAU - Kim, M-J
AU  - Kim MJ
FAU - Ha, E
AU  - Ha E
FAU - Chung, J-H
AU  - Chung JH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070925
PL  - Germany
TA  - Phytomedicine
JT  - Phytomedicine : international journal of phytotherapy and phytopharmacology
JID - 9438794
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
RN  - 0 (RNA, Messenger)
RN  - 0 (bcl-2-Associated X Protein)
RN  - E750O06Y6O (Hesperidin)
RN  - EC 3.4.22.- (Caspase 3)
SB  - IM
MH  - Apoptosis/*drug effects/genetics
MH  - Caspase 3/*metabolism
MH  - Cell Line, Tumor
MH  - Cell Survival/drug effects
MH  - Citrus/chemistry
MH  - Colonic Neoplasms/*drug therapy/genetics/*metabolism
MH  - Enzyme Activation/drug effects
MH  - Gene Expression Regulation, Neoplastic/drug effects
MH  - Hesperidin/chemistry/*pharmacology
MH  - Humans
MH  - Molecular Structure
MH  - Proto-Oncogene Proteins c-bcl-2/genetics
MH  - RNA, Messenger/metabolism
MH  - bcl-2-Associated X Protein/genetics
EDAT- 2007/09/28 09:00
MHDA- 2008/05/31 09:00
CRDT- 2007/09/28 09:00
PHST- 2007/09/28 09:00 [pubmed]
PHST- 2008/05/31 09:00 [medline]
PHST- 2007/09/28 09:00 [entrez]
AID - S0944-7113(07)00185-7 [pii]
AID - 10.1016/j.phymed.2007.07.061 [doi]
PST - ppublish
SO  - Phytomedicine. 2008 Jan;15(1-2):147-51. doi: 10.1016/j.phymed.2007.07.061. Epub 
      2007 Sep 25.