PMID- 17897884
OWN - NLM
STAT- MEDLINE
DCOM- 20080708
LR  - 20220318
IS  - 1618-0607 (Electronic)
IS  - 1438-4221 (Linking)
VI  - 298
IP  - 5-6
DP  - 2008 Jul
TI  - Imbalance in intestinal microflora constitution could be involved in the 
      pathogenesis of inflammatory bowel disease.
PG  - 463-72
AB  - Since genetically engineered animal models of inflammatory bowel disease (IBD) do 
      not develop colitis under germ-free conditions, the intestinal microflora is 
      thought to be one of the most important environmental factors associated with 
      IBD. To understand the involvement of intestinal microflora in the pathogenesis 
      of IBD, we analyzed the constituents of intestinal microflora in IBD. Faecal 
      samples from 73 patients with ulcerative colitis (UC) and 23 patients with 
      Crohn's disease (CD) were analyzed by quantitative PCR using 16S rRNA 
      gene-targeted group-specific primers for Bacteroides fragilis group, 
      Bifidobacterium, Clostridium coccoides groups, Clostridium leptum subgroup, 
      Atopobium cluster, and seven species of Bacteroides. We analyzed the distribution 
      of the predominant microflora by fluorescence in situ hybridization (FISH) using 
      group-specific probes. We also examined the concentration of faecal organic acids 
      produced by intestinal microflora. Contrary to previous reports, we found that 
      the B. fragilis group was significantly decreased in the faeces of patients with 
      IBD. Moreover, B. vulgatus was the predominant microflora in healthy controls and 
      relatively decreased among IBD patients. Most of the microflora adhering to the 
      colonic mucosa surrounding the mucus layer comprised C. coccoides group and 
      Bifidobacterium. B. fragilis group mainly inhabited the faeces, but did not 
      adhere to or invade the mucosa. The concentrations of propionic and butyric acids 
      in the faeces were significantly decreased in patients with IBD. These findings 
      indicate that IBD is not caused by a specific intestinal bacterial cluster or 
      species and that disordered intestinal microflora could be involved in the 
      pathogenesis of IBD.
FAU - Takaishi, Hiromasa
AU  - Takaishi H
AD  - Division of Gastroenterology and Hepatology, Department of Internal Medicine, 
      Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, 
      Japan.
FAU - Matsuki, Takahiro
AU  - Matsuki T
FAU - Nakazawa, Atsushi
AU  - Nakazawa A
FAU - Takada, Toshihiko
AU  - Takada T
FAU - Kado, Shoichi
AU  - Kado S
FAU - Asahara, Takashi
AU  - Asahara T
FAU - Kamada, Nobuhiko
AU  - Kamada N
FAU - Sakuraba, Atsushi
AU  - Sakuraba A
FAU - Yajima, Tomoharu
AU  - Yajima T
FAU - Higuchi, Hajime
AU  - Higuchi H
FAU - Inoue, Nagamu
AU  - Inoue N
FAU - Ogata, Haruhiko
AU  - Ogata H
FAU - Iwao, Yasushi
AU  - Iwao Y
FAU - Nomoto, Koji
AU  - Nomoto K
FAU - Tanaka, Ryuichiro
AU  - Tanaka R
FAU - Hibi, Toshifumi
AU  - Hibi T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070925
PL  - Germany
TA  - Int J Med Microbiol
JT  - International journal of medical microbiology : IJMM
JID - 100898849
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Bacterial)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (Propionates)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 107-92-6 (Butyric Acid)
RN  - JHU490RVYR (propionic acid)
SB  - IM
MH  - Actinobacteria/classification/genetics/isolation & purification
MH  - Bacteria/*classification/genetics/*isolation & purification
MH  - Bacteroides/classification/genetics/isolation & purification
MH  - Bifidobacterium/classification/genetics/isolation & purification
MH  - *Biodiversity
MH  - Butyric Acid/analysis
MH  - Clostridium/classification/genetics/isolation & purification
MH  - Colony Count, Microbial/methods
MH  - DNA Primers/genetics
MH  - DNA, Bacterial/genetics
MH  - DNA, Ribosomal/genetics
MH  - Feces/chemistry/microbiology
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Inflammatory Bowel Diseases/*microbiology
MH  - Intestinal Mucosa/microbiology
MH  - Intestines/*microbiology
MH  - Polymerase Chain Reaction/methods
MH  - Propionates/analysis
MH  - RNA, Ribosomal, 16S/genetics
EDAT- 2007/09/28 09:00
MHDA- 2008/07/09 09:00
CRDT- 2007/09/28 09:00
PHST- 2006/12/22 00:00 [received]
PHST- 2007/07/19 00:00 [revised]
PHST- 2007/07/31 00:00 [accepted]
PHST- 2007/09/28 09:00 [pubmed]
PHST- 2008/07/09 09:00 [medline]
PHST- 2007/09/28 09:00 [entrez]
AID - S1438-4221(07)00145-2 [pii]
AID - 10.1016/j.ijmm.2007.07.016 [doi]
PST - ppublish
SO  - Int J Med Microbiol. 2008 Jul;298(5-6):463-72. doi: 10.1016/j.ijmm.2007.07.016. 
      Epub 2007 Sep 25.