PMID- 17902174
OWN - NLM
STAT- MEDLINE
DCOM- 20080325
LR  - 20131121
IS  - 1554-527X (Electronic)
IS  - 0736-0266 (Linking)
VI  - 26
IP  - 3
DP  - 2008 Mar
TI  - Ischemia/reperfusion-induced necrosis and apoptosis in the cells isolated from 
      rat skeletal muscle.
PG  - 351-6
AB  - Necrosis was considered to be the solo mechanism for ischemia/reperfusion 
      (I/R)-induced cell death. Recent evidence from I/R models of the heart, liver, 
      kidney, and brain indicates that apoptosis is a major contributor to I/R-induced 
      cell death. However, evidence of I/R-induced apoptosis in skeletal muscle is 
      sparse and divided. The purpose for the present study was to investigate 
      I/R-induced necrosis and apoptosis in the cells isolated from rat skeletal 
      muscle. A rat gracilis muscle model was used. After surgical preparation, clamps 
      were applied on the vascular pedicle to create 4 h of ischemia and released for 
      24 h of reperfusion (I/R, n = 10). Clamping was omitted in sham I/R rats (sham 
      I/R, n = 10). The muscle samples were harvested after 24 h of reperfusion for the 
      process of cell isolation. Cells were stained by Propidium Iodide (PI) or Annexin 
      V-FITC or both. Twenty thousand cells from each muscle sample were scanned and 
      analyzed by flow cytometry. The average percentage of live cells was 45 +/- 2% in 
      the I/R group versus 65 +/- 3% in the sham I/R group (p < 0.01). The average 
      percentage of necrotic cells was 18 +/- 1% in I/R versus 12 +/- 1% in sham I/R (p 
      < 0.01). The average percentage of apoptotic cells was 40 +/- 3% in I/R versus 27 
      +/- 3% in sham I/R (p < 0.01). Our results clearly demonstrated that I/R not only 
      causes necrosis, but also accelerates apoptosis in the cells isolated from rat 
      skeletal muscle.
CI  - Copyright 2007 Orthopaedic Research Society. Published by Wiley Periodicals, Inc.
FAU - Wang, Wei Z
AU  - Wang WZ
AD  - Department of Surgery, University of Nevada School of Medicine, 2040 W. 
      Charleston Blvd., Suite 301, Las Vegas, Nevada 89102, USA. wzwang2000@yahoo.com
FAU - Fang, Xin-Hua
AU  - Fang XH
FAU - Stephenson, Linda L
AU  - Stephenson LL
FAU - Khiabani, Kayvan T
AU  - Khiabani KT
FAU - Zamboni, William A
AU  - Zamboni WA
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Orthop Res
JT  - Journal of orthopaedic research : official publication of the Orthopaedic 
      Research Society
JID - 8404726
RN  - 0 (Annexin A5)
RN  - 0 (Coloring Agents)
RN  - 0 (Drug Combinations)
RN  - 0 (Fluorescent Dyes)
RN  - 36015-30-2 (Propidium)
RN  - I223NX31W9 (Fluorescein-5-isothiocyanate)
SB  - IM
MH  - Animals
MH  - Annexin A5
MH  - *Apoptosis
MH  - Cell Separation
MH  - Coloring Agents
MH  - Drug Combinations
MH  - Flow Cytometry
MH  - Fluorescein-5-isothiocyanate
MH  - Fluorescent Dyes
MH  - Male
MH  - Muscle, Skeletal/*blood supply/*pathology
MH  - Necrosis
MH  - Propidium
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reperfusion Injury/*pathology/*physiopathology
MH  - Staining and Labeling
EDAT- 2007/09/29 09:00
MHDA- 2008/03/26 09:00
CRDT- 2007/09/29 09:00
PHST- 2007/09/29 09:00 [pubmed]
PHST- 2008/03/26 09:00 [medline]
PHST- 2007/09/29 09:00 [entrez]
AID - 10.1002/jor.20493 [doi]
PST - ppublish
SO  - J Orthop Res. 2008 Mar;26(3):351-6. doi: 10.1002/jor.20493.