PMID- 17904197
OWN - NLM
STAT- MEDLINE
DCOM- 20080414
LR  - 20240109
IS  - 0045-6535 (Print)
IS  - 0045-6535 (Linking)
VI  - 70
IP  - 6
DP  - 2008 Jan
TI  - Comparison of the removal of 2,4-dichlorophenol and phenol from polluted water, 
      by peroxidases from tomato hairy roots, and protective effect of polyethylene 
      glycol.
PG  - 982-9
AB  - Multiple efforts have been directed towards optimized processes in which enzymes, 
      like peroxidases, are used to remove phenolic compounds from polluted wastewater. 
      Here we describe the use of peroxidase isoenzymes from tomato hairy roots, which 
      were able to oxidise 2,4-dichlorophenol (2,4-DCP) and phenol from aqueous 
      solutions. This could be an interesting alternative for the removal of these 
      compounds from contaminated sites. We used different enzyme fractions: total 
      peroxidases (TP), ionically bound to cell wall peroxidases (IBP), basic (BP) and 
      acidic peroxidases (AP). We analyzed the optimum conditions of removal, the 
      effect of Polyethyleneglycol (PEG-3350) on the process and on the enzyme 
      activities, to obtain the maximum efficiency. The optimal H2O2 concentrations for 
      2,4-DCP and phenol removal were 1 and 0.1mM, respectively. TP, IBP and BP showed 
      better removal efficiencies than AP, for both contaminants. The addition of 
      different concentrations (10-100mg l(-1)) of PEG-3350 to solutions containing 
      2,4-DCP showed no effect on the removal efficiencies of the isoenzymes. However, 
      PEG (100mg l(-1)) increased the removal efficiency of phenol by BP and IBP 
      fractions. On the other hand, peroxidase activities from BP and IBP fractions 
      were 3 and 13 times higher, respectively, than those detected for the same 
      fractions in phenol treated solutions without PEG. The protective effect of PEG, 
      which depends on the contaminant as well as of the enzyme fraction used, would be 
      important to improve the removal efficiency of phenol by some peroxidase 
      isoenzymes.
FAU - Gonzalez, Paola Solange
AU  - Gonzalez PS
AD  - Departamento de Biologia Molecular, FCEFQN, Universidad Nacional de Rio Cuarto, 
      5800 Rio Cuarto, Cordoba, Argentina.
FAU - Agostini, Elizabeth
AU  - Agostini E
FAU - Milrad, Silvia Raquel
AU  - Milrad SR
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070927
PL  - England
TA  - Chemosphere
JT  - Chemosphere
JID - 0320657
RN  - 0 (Chlorophenols)
RN  - 0 (Isoenzymes)
RN  - 0 (Plant Proteins)
RN  - 0 (Water Pollutants, Chemical)
RN  - 339NCG44TV (Phenol)
RN  - 3WJQ0SDW1A (Polyethylene Glycols)
RN  - EC 1.11.1.7 (Peroxidase)
RN  - R669TG1950 (2,4-dichlorophenol)
SB  - IM
MH  - Chlorophenols/*isolation & purification/metabolism
MH  - Enzyme Activation/drug effects
MH  - Isoenzymes/metabolism
MH  - Peroxidase/*metabolism
MH  - Phenol/*isolation & purification/metabolism
MH  - Plant Proteins/metabolism
MH  - Plant Roots/*enzymology
MH  - Polyethylene Glycols/pharmacology
MH  - Water Pollutants, Chemical/*isolation & purification
MH  - Water Pollution, Chemical/prevention & control
EDAT- 2007/10/02 09:00
MHDA- 2008/04/15 09:00
CRDT- 2007/10/02 09:00
PHST- 2007/04/16 00:00 [received]
PHST- 2007/08/03 00:00 [revised]
PHST- 2007/08/07 00:00 [accepted]
PHST- 2007/10/02 09:00 [pubmed]
PHST- 2008/04/15 09:00 [medline]
PHST- 2007/10/02 09:00 [entrez]
AID - S0045-6535(07)01015-6 [pii]
AID - 10.1016/j.chemosphere.2007.08.025 [doi]
PST - ppublish
SO  - Chemosphere. 2008 Jan;70(6):982-9. doi: 10.1016/j.chemosphere.2007.08.025. Epub 
      2007 Sep 27.