PMID- 17905524
OWN - NLM
STAT- MEDLINE
DCOM- 20071228
LR  - 20131121
IS  - 0344-0338 (Print)
IS  - 0344-0338 (Linking)
VI  - 203
IP  - 11
DP  - 2007
TI  - Percutaneous coronary injection of bone marrow cells in small experimental 
      animals: small is not too small.
PG  - 801-8
AB  - Intracoronary infusion of bone marrow cells (BMCs) is thought to induce cardiac 
      regeneration in ischemic heart disease and dilated cardiomyopathy. The aim of our 
      study was to develop a new method to inject BMCs into coronary arteries of small 
      experimental animals. Transient atrioventricular block (AVB) was induced in 25 
      rats and 39 hamsters by intracarotid injection of adenosine 5'-triphosphate 
      (ATP). Contrast echocardiography was obtained. BMCs (0.2-0.5 ml) were collected 
      through femoral puncture, stained with PKH26 and injected into the carotid artery 
      (CA). Animals were immediately sacrificed or followed for 1 month. To evaluate 
      BMCs transfer from CA to myocardium, AVB and BMCs injections were performed in 10 
      hamsters subjected to coronary ligation for 30 min. Induction of transient AVB 
      was possible in all animals by injecting 20-30 mg of ATP. Animals recovered a 
      basal cardiac activity spontaneously or by dopamine injection. Flash injection of 
      contrast medium through the CA induced staining of aortic root, coronary 
      arteries, and myocardium. BMCs injection was possible in all cases. No immediate 
      or late ECG changes were observed. Immediately after injection in healthy 
      animals, histological examination showed the presence of BMCs in small coronary 
      arteries and, after 1 month, the absence of infarction. In ischemic hearts, the 
      presence of BMCs in the myocardium was observed 24h after ischemia. ATP-induced 
      AVB block allows for percutaneous intracoronary injection of BMCs in small 
      experimental animals with no immediate or late mortality and morbidity. This 
      method offers new perspectives for the investigation of BMCs coronary infusion 
      and engraftment in heart diseases.
FAU - Agnoletti, Gabriella
AU  - Agnoletti G
AD  - Necker Enfants Malades, Paris, France. gabriella.agnoletti@nck.ap-hop-paris.fr
FAU - Cargnoni, Anna
AU  - Cargnoni A
FAU - Agnoletti, Laura
AU  - Agnoletti L
FAU - Di Marcello, Marco
AU  - Di Marcello M
FAU - Balzarini, Piera
AU  - Balzarini P
FAU - Gitti, Luca
AU  - Gitti L
FAU - Martina, Patrizia
AU  - Martina P
FAU - Grigolato, Pier Giovanni
AU  - Grigolato PG
FAU - Ferrari, Roberto
AU  - Ferrari R
LA  - eng
PT  - Journal Article
DEP - 20071001
PL  - Germany
TA  - Pathol Res Pract
JT  - Pathology, research and practice
JID - 7806109
RN  - 8L70Q75FXE (Adenosine Triphosphate)
SB  - IM
MH  - Adenosine Triphosphate/toxicity
MH  - Administration, Cutaneous
MH  - Animals
MH  - Atrioventricular Block/chemically induced
MH  - Bone Marrow Transplantation/*methods
MH  - *Carotid Arteries
MH  - *Coronary Vessels
MH  - Cricetinae
MH  - Echocardiography
MH  - Flow Cytometry
MH  - Male
MH  - Mesocricetus
MH  - Myocardial Ischemia/*therapy
MH  - Myocardium/*cytology
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 2007/10/02 09:00
MHDA- 2007/12/29 09:00
CRDT- 2007/10/02 09:00
PHST- 2007/03/26 00:00 [received]
PHST- 2007/07/04 00:00 [revised]
PHST- 2007/07/20 00:00 [accepted]
PHST- 2007/10/02 09:00 [pubmed]
PHST- 2007/12/29 09:00 [medline]
PHST- 2007/10/02 09:00 [entrez]
AID - S0344-0338(07)00164-1 [pii]
AID - 10.1016/j.prp.2007.07.008 [doi]
PST - ppublish
SO  - Pathol Res Pract. 2007;203(11):801-8. doi: 10.1016/j.prp.2007.07.008. Epub 2007 
      Oct 1.