PMID- 17907002
OWN - NLM
STAT- MEDLINE
DCOM- 20080408
LR  - 20171116
IS  - 1071-5762 (Print)
IS  - 1029-2470 (Linking)
VI  - 41
IP  - 11
DP  - 2007 Nov
TI  - Polypeptide from Chlamys farreri inhibits UVB-induced HaCaT cells apoptosis via 
      inhibition CD95 pathway and reactive oxygen species.
PG  - 1224-32
AB  - Polypeptide from Chlamys farreri (PCF) is a novel marine active product isolated 
      from gonochoric Chinese scallop Chlamys farreri which has recently been found to 
      be an effective antioxidant. In this study, we assessed the effect of PCF on 
      UVB-induced intracellular signalling of apoptosis in HaCaT cells. Pre-treatment 
      with PCF significantly inhibited UVB-induced apoptosis in HaCaT cells. PCF 
      strongly reduced the intracellular reactive oxygen species (ROS) level followed 
      by inhibiting the release of cytochrome c. The expression of CD95 and 
      Fas-associating protein with death domain (FADD) was eliminated in a 
      dose-dependent manner by PCF pre-treatment in UVB-irradiated HaCaT cells, 
      followed by inhibition of cleavage of procaspase-8, whose activation induced cell 
      apoptosis. Furthermore, pre-treatment with the ROS scavenger N-acetylcysteine 
      (NAC) and the caspase-8 inhibitor z-IETD-fmk was found to effectively prevent 
      UVB-induced apoptosis, suggesting that UVB-induced HaCaT cell apoptosis was 
      partially due to generation of ROS and activation of the caspase-8 pathway. 
      Consequently, the protective effect of PCF against UVB irradiation in HaCaT cells 
      is exerted by suppression of generation of ROS followed by inhibition of 
      cytochrome c release and inactivation of Fas-FADD-caspase-8 pathway, resulting in 
      blockage of UVB-induced apoptosis.
FAU - Li, Bing-Hua
AU  - Li BH
AD  - Department of Pharmacology, Medical College, Qingdao University, Qingdao, 
      Shandong 266021, China. bhli666@126.com
FAU - Zhou, Ying-Bin
AU  - Zhou YB
FAU - Guo, Shen-Bo
AU  - Guo SB
FAU - Wang, Chun-bo
AU  - Wang CB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Free Radic Res
JT  - Free radical research
JID - 9423872
RN  - 0 (Fas-Associated Death Domain Protein)
RN  - 0 (Free Radical Scavengers)
RN  - 0 (Peptides)
RN  - 0 (RNA, Messenger)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (fas Receptor)
RN  - WYQ7N0BPYC (Acetylcysteine)
SB  - IM
MH  - Acetylcysteine/pharmacology
MH  - Animals
MH  - Apoptosis/drug effects/radiation effects
MH  - Cells, Cultured
MH  - Drug Evaluation, Preclinical
MH  - Fas-Associated Death Domain Protein/genetics
MH  - Free Radical Scavengers/pharmacology
MH  - Gene Expression/drug effects/radiation effects
MH  - Humans
MH  - Keratinocytes/*drug effects/metabolism/physiology/*radiation effects
MH  - Pectinidae/*chemistry
MH  - Peptides/isolation & purification/*pharmacology
MH  - RNA, Messenger/metabolism
MH  - Reactive Oxygen Species/*antagonists & inhibitors/metabolism
MH  - Signal Transduction/drug effects
MH  - Ultraviolet Rays
MH  - fas Receptor/*antagonists & inhibitors/genetics
EDAT- 2007/10/02 09:00
MHDA- 2008/04/09 09:00
CRDT- 2007/10/02 09:00
PHST- 2007/10/02 09:00 [pubmed]
PHST- 2008/04/09 09:00 [medline]
PHST- 2007/10/02 09:00 [entrez]
AID - 782728473 [pii]
AID - 10.1080/10715760701636858 [doi]
PST - ppublish
SO  - Free Radic Res. 2007 Nov;41(11):1224-32. doi: 10.1080/10715760701636858.