PMID- 17908300 OWN - NLM STAT- MEDLINE DCOM- 20080117 LR - 20211020 IS - 1472-6807 (Electronic) IS - 1472-6807 (Linking) VI - 7 DP - 2007 Oct 1 TI - X-ray structures of two proteins belonging to Pfam DUF178 revealed unexpected structural similarity to the DUF191 Pfam family. PG - 62 AB - BACKGROUND: Pfam is a comprehensive collection of protein domains and families, with a range of well-established information including genome annotation. Pfam has two large series of functionally uncharacterized families, known as Domains of Unknown Function (DUFs) and Uncharacterized Protein Families (UPFs). RESULTS: Crystal structures of two proteins from Deinococcus radiodurans and Streptomyces coelicolor belonging to Pfam protein family DUF178 (ID: PF02621) have been determined using Selenium-Single-wavelength Anomalous Dispersion (Se-SAD). Based on the structure, we have identified the putative function for this family of protein. CONCLUSION: Unexpectedly, we found that DUF178 Pfam is remarkably similar to Pfam family DUF191 suggesting that the sequence-based classification alone may not be sufficient to classify proteins into Pfam families. FAU - Tyagi, Rajiv AU - Tyagi R AD - Biology Department, Brookhaven National Laboratory, Upton, New York 11973, USA. swami@bnl.gov FAU - Burley, Stephen K AU - Burley SK FAU - Swaminathan, Subramanyam AU - Swaminathan S LA - eng GR - U54 GM074945/GM/NIGMS NIH HHS/United States GR - GM074945/GM/NIGMS NIH HHS/United States PT - Journal Article PT - Research Support, N.I.H., Extramural PT - Research Support, U.S. Gov't, Non-P.H.S. DEP - 20071001 PL - England TA - BMC Struct Biol JT - BMC structural biology JID - 101088689 RN - 0 (Bacterial Proteins) RN - 0 (Ligands) SB - IM MH - Amino Acid Sequence MH - Bacterial Proteins/*chemistry/*classification MH - Binding Sites MH - Crystallography, X-Ray MH - Deinococcus/*chemistry MH - Ligands MH - Molecular Sequence Data MH - Protein Structure, Secondary MH - Protein Structure, Tertiary MH - Sequence Alignment MH - Sequence Analysis, Protein MH - Sequence Homology, Amino Acid MH - Streptomyces coelicolor/*chemistry MH - *Structural Homology, Protein PMC - PMC2174479 EDAT- 2007/10/03 09:00 MHDA- 2008/01/18 09:00 PMCR- 2007/10/01 CRDT- 2007/10/03 09:00 PHST- 2007/05/31 00:00 [received] PHST- 2007/10/01 00:00 [accepted] PHST- 2007/10/03 09:00 [pubmed] PHST- 2008/01/18 09:00 [medline] PHST- 2007/10/03 09:00 [entrez] PHST- 2007/10/01 00:00 [pmc-release] AID - 1472-6807-7-62 [pii] AID - 10.1186/1472-6807-7-62 [doi] PST - epublish SO - BMC Struct Biol. 2007 Oct 1;7:62. doi: 10.1186/1472-6807-7-62.