PMID- 17908424
OWN - NLM
STAT- MEDLINE
DCOM- 20111104
LR  - 20131121
IS  - 1001-9391 (Print)
IS  - 1001-9391 (Linking)
VI  - 25
IP  - 7
DP  - 2007 Jul
TI  - [c-Jun NH2-terminal kinase and extracellular signal-regulated protein kinase 
      signaling pathways in regulation of benzo(a)pyrene-induced c-Jun activation in 
      human embryo lung fibroblasts].
PG  - 385-8
AB  - OBJECTIVE: To investigate the role of mitogen activated protein kinases (MAPKs) 
      signaling pathways in the regulation of benzo(a)pyrene (B(a)P)-induced c-Jun 
      activation in human embryo lung fibroblasts (HELFs). METHODS: HELFs were cultured 
      with 2.0 micromol/L B(a)P for various time (0, 3, 6, 12, 24 h) or with various 
      concentration of B(a)P (0.0, 0.5, 1.0, 2.0 micromol/L) for 12 h. Western blot was 
      performed to examine the effect of B(a)P on c-Jun activation. The dominant 
      negative mutants of p38, c-Jun NH2-terminal kinase (JNK) and extracellular 
      signal-regulated protein kinase (ERK) were applied to establish stable 
      transfectant, and to detect the relationship of MAPK signal molecules and c-Jun 
      activation in B (a) P-treated cells. RESULTS: B(a)P treatment resulted in a 
      marked activation of c-Jun in time-dependent manner with a peak at 12 h (the 
      densitometric ratios of phosphorylated c-Jun Ser63, Ser73 to actin were 20.1, 
      15.2 times for control respectively) and in dose-dependent manner. However, there 
      was no evident change on total c-Jun expression in B(a)P-treated HELFs. Moreover, 
      B(a)P-induced activation of c-Jun was inhibited by stable expression of dominant 
      negative mutants of JNK or ERK, but not by dominant negative mutant of p38. 
      CONCLUSION: JNK and ERK signaling pathways, but not p38 pathway regulate 
      B(a)P-induced c-Jun activation in HELFs.
FAU - Jiao, Shi
AU  - Jiao S
AD  - National Institute of Occupation Health and Poison Control, Chinese Center for 
      Disease Control and Prevention, Beijing 100050, China.
FAU - Liu, Bing-ci
AU  - Liu BC
FAU - Shi, Xiang-lin
AU  - Shi XL
FAU - Huang, Chuan-shu
AU  - Huang CS
FAU - Gao, Ai
AU  - Gao A
FAU - Ye, Meng
AU  - Ye M
FAU - Jia, Xiao-wei
AU  - Jia XW
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi
JT  - Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng 
      zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases
JID - 8410840
RN  - 0 (Proto-Oncogene Proteins c-jun)
RN  - 3417WMA06D (Benzo(a)pyrene)
RN  - EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases)
RN  - EC 2.7.11.24 (JNK Mitogen-Activated Protein Kinases)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
SB  - IM
MH  - Benzo(a)pyrene/*pharmacology
MH  - Cells, Cultured
MH  - Embryo, Mammalian/cytology
MH  - Extracellular Signal-Regulated MAP Kinases/*metabolism
MH  - Fibroblasts/drug effects/*metabolism
MH  - Humans
MH  - JNK Mitogen-Activated Protein Kinases/*metabolism
MH  - Lung/cytology/metabolism
MH  - Phosphorylation/drug effects
MH  - Proto-Oncogene Proteins c-jun/*metabolism
MH  - Signal Transduction/drug effects
MH  - p38 Mitogen-Activated Protein Kinases/metabolism
EDAT- 2007/10/03 09:00
MHDA- 2011/11/05 06:00
CRDT- 2007/10/03 09:00
PHST- 2007/10/03 09:00 [pubmed]
PHST- 2011/11/05 06:00 [medline]
PHST- 2007/10/03 09:00 [entrez]
PST - ppublish
SO  - Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2007 Jul;25(7):385-8.