PMID- 17912627
OWN - NLM
STAT- MEDLINE
DCOM- 20080807
LR  - 20211020
IS  - 0272-4340 (Print)
IS  - 0272-4340 (Linking)
VI  - 28
IP  - 4
DP  - 2008 Jun
TI  - Melatonin regulates the viability and differentiation of rat midbrain neural stem 
      cells.
PG  - 569-79
AB  - (1) Neurogenesis driven by neural stem cells (NSCs) is regulated by physiological 
      and pathological factors. Melatonin (MT) has profound neurotrophic and 
      neuroprotective effects. Hence, we studied the role of MT in regulating the 
      viability and differentiation of NSCs derived from rat ventral midbrain. (2) NSCs 
      were isolated from the rat ventral midbrain. The viability of NSCs was determined 
      by 
      3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-ulfophenyl)-2H-tetrazolium 
      assay. The differentiation of NSCs was examined by analyzing the expression of 
      the neural markers, MT receptors, brain derived neurotropic factor (BDNF) and 
      glial cell line-derived neurotrophic factor (GDNF) with semi-quantitative RT-PCR, 
      immunofluorescence cytochemistry, and Western blot. (3) Our results showed that 
      MT could promote the viability of NSCs. In addition, MT could significantly 
      elevate the mRNA and protein levels of tyroxine hydroxylase (TH), a marker of 
      dopaminergic neurons, and decrease the expression of the astrocytes maker glial 
      fibrillary acidic protein (GFAP). MT also increased the production of BDNF and 
      GDNF in the cultured NSCs. Meanwhile, we first found that two subtypes of MT 
      receptors, MT1 and MT2, were expressed in the ventral midbrain NSCs. (4) These 
      results demonstrated that MT could induce NSCs to differentiate into dopaminergic 
      neurons and decrease astrocyte production. These findings also suggest that MT 
      could offer a beneficial tool in guiding directional differentiation of NSCs.
FAU - Kong, Xiangying
AU  - Kong X
AD  - Department of Pharmacology, School of Basic Medicine, Peking Union Medical 
      College and Institute of Basic Medical Science, Chinese Academy of Medical 
      Sciences, 5 Dong Dan San Tiao, Beijing 100005, China.
FAU - Li, Xuekun
AU  - Li X
FAU - Cai, Zhe
AU  - Cai Z
FAU - Yang, Nan
AU  - Yang N
FAU - Liu, Yanyong
AU  - Liu Y
FAU - Shu, Jun
AU  - Shu J
FAU - Pan, Lin
AU  - Pan L
FAU - Zuo, Pingping
AU  - Zuo P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20071003
PL  - United States
TA  - Cell Mol Neurobiol
JT  - Cellular and molecular neurobiology
JID - 8200709
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Receptor, Melatonin, MT1)
RN  - 0 (Receptor, Melatonin, MT2)
RN  - JL5DK93RCL (Melatonin)
RN  - VTD58H1Z2X (Dopamine)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/genetics/metabolism
MH  - Cell Differentiation/*drug effects
MH  - Cell Survival/drug effects
MH  - Cells, Cultured
MH  - Dopamine/metabolism
MH  - Embryo, Mammalian
MH  - Embryonic Stem Cells/*drug effects/metabolism/physiology
MH  - Female
MH  - Gene Expression Regulation/drug effects
MH  - Melatonin/*pharmacology
MH  - Mesencephalon/*drug effects/metabolism/physiology
MH  - Neurons/*drug effects/metabolism/physiology
MH  - Pregnancy
MH  - Rats
MH  - Receptor, Melatonin, MT1/genetics/metabolism
MH  - Receptor, Melatonin, MT2/genetics/metabolism
EDAT- 2007/10/04 09:00
MHDA- 2008/08/08 09:00
CRDT- 2007/10/04 09:00
PHST- 2007/05/06 00:00 [received]
PHST- 2007/08/30 00:00 [accepted]
PHST- 2007/10/04 09:00 [pubmed]
PHST- 2008/08/08 09:00 [medline]
PHST- 2007/10/04 09:00 [entrez]
AID - 10.1007/s10571-007-9212-7 [doi]
PST - ppublish
SO  - Cell Mol Neurobiol. 2008 Jun;28(4):569-79. doi: 10.1007/s10571-007-9212-7. Epub 
      2007 Oct 3.