PMID- 17913746
OWN - NLM
STAT- MEDLINE
DCOM- 20071227
LR  - 20211020
IS  - 1362-4962 (Electronic)
IS  - 0305-1048 (Print)
IS  - 0305-1048 (Linking)
VI  - 35
IP  - 19
DP  - 2007
TI  - The ePHD protein SPBP interacts with TopBP1 and together they co-operate to 
      stimulate Ets1-mediated transcription.
PG  - 6648-62
AB  - SPBP (Stromelysin-1 PDGF responsive element binding protein) is a ubiquitously 
      expressed 220 kDa nuclear protein shown to enhance or repress the transcriptional 
      activity of various transcription factors. A yeast two-hybrid screen, with the 
      extended plant homeodomain (ePHD) of SPBP as bait, identified TopBP1 
      (topoisomerase II beta-binding protein 1) as a candidate interaction partner of 
      SPBP. TopBP1 has eight BRCA1 carboxy-terminal (BRCT) domains and is involved in 
      DNA replication, DNA damage responses and in the regulation of gene expression. 
      The interaction between SPBP and TopBP1 was confirmed in vitro and in vivo, and 
      was found to be mediated by the ePHD domain of SPBP and the BRCT6 domain of 
      TopBP1. Both SPBP and TopBP1 enhanced the transcriptional activity of Ets1 on the 
      c-myc P1P2- and matrix metalloproteinase-3 (MMP3) promoters. Together they 
      displayed a more than additive effect. Both proteins were associated with these 
      promoters. The involvement of TopBP1 was dependent on the serine 1159 
      phosphorylation site, known to be important for transcriptional activation. 
      Depletion of endogenous SPBP by siRNA treatment reduced MMP3 secretion by 50% in 
      phorbol ester-stimulated human fibroblasts. Taken together, our results show that 
      TopBP1 and SPBP interact physically and functionally to co-operate as 
      co-activators of Ets1.
FAU - Sjottem, Eva
AU  - Sjottem E
AD  - Biochemistry Department and Department of Molecular Biotechnology, Institute of 
      Medical Biology, University of Tromso, 9037 Tromso, Norway.
FAU - Rekdal, Cecilie
AU  - Rekdal C
FAU - Svineng, Gunbjorg
AU  - Svineng G
FAU - Johnsen, Sylvia Sagen
AU  - Johnsen SS
FAU - Klenow, Helle
AU  - Klenow H
FAU - Uglehus, Rebecca Dale
AU  - Uglehus RD
FAU - Johansen, Terje
AU  - Johansen T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20071002
PL  - England
TA  - Nucleic Acids Res
JT  - Nucleic acids research
JID - 0411011
RN  - 0 (Carrier Proteins)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Proto-Oncogene Protein c-ets-1)
RN  - 0 (RAI1 protein, human)
RN  - 0 (TCF20 protein, human)
RN  - 0 (TOPBP1 protein, human)
RN  - 0 (Trans-Activators)
RN  - 0 (Transcription Factors)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - Carrier Proteins/chemistry/*metabolism
MH  - DNA-Binding Proteins/chemistry/*metabolism
MH  - Fibroblasts/enzymology
MH  - HeLa Cells
MH  - Humans
MH  - Matrix Metalloproteinase 3/genetics/metabolism
MH  - Nuclear Proteins/chemistry/*metabolism
MH  - Phosphorylation
MH  - Promoter Regions, Genetic
MH  - Protein Structure, Tertiary
MH  - Proto-Oncogene Protein c-ets-1/*metabolism
MH  - Trans-Activators
MH  - Transcription Factors/chemistry/*metabolism
MH  - *Transcriptional Activation
MH  - Two-Hybrid System Techniques
PMC - PMC2095823
EDAT- 2007/10/05 09:00
MHDA- 2007/12/28 09:00
PMCR- 2007/10/02
CRDT- 2007/10/05 09:00
PHST- 2007/10/05 09:00 [pubmed]
PHST- 2007/12/28 09:00 [medline]
PHST- 2007/10/05 09:00 [entrez]
PHST- 2007/10/02 00:00 [pmc-release]
AID - gkm739 [pii]
AID - 10.1093/nar/gkm739 [doi]
PST - ppublish
SO  - Nucleic Acids Res. 2007;35(19):6648-62. doi: 10.1093/nar/gkm739. Epub 2007 Oct 2.