PMID- 17914193
OWN - NLM
STAT- MEDLINE
DCOM- 20071109
LR  - 20211020
IS  - 1073-6085 (Print)
IS  - 1073-6085 (Linking)
VI  - 36
IP  - 2
DP  - 2007 Jun
TI  - Construction of microarrays for genotyping of DQA using unmodified 45-mer 
      oligonucleotide.
PG  - 142-50
AB  - The human leukocyte antigen (HLA) class II system is strongly connected to 
      immunological response and its compatibility between tissues is critical in 
      transplantation. The simple robust typing analyses of HLA genes are extremely 
      important. In this paper, we developed an approach based on microarray technology 
      for genotyping of DQA gene. The microarrays were constructed with a total 31 
      unmodified 45-mer oligonucleotide. The second exon of DQA gene was amplified, and 
      allowed to hybridize with the array. DQA genotypes were assigned by quantitative 
      analysis of the hybridization results. The arrays were evaluated by DQA 
      genotyping of nine reference samples and 120 clinical samples. The results 
      demonstrate that the genotyping accuracy/concordance achieved 97.5% compared with 
      the direct DNA sequencing. Although our methods did not perform high-resolution 
      genotyping, it could be an alternative for serological typing in routine medical 
      practice.
FAU - Yin, Bin-Cheng
AU  - Yin BC
AD  - State Key Laboratory of Bioreactor Engineering, Department of Food Engineering 
      and Science, East China University of Science & Technology, Shanghai 200237, 
      China.
FAU - Yue, Fei
AU  - Yue F
FAU - Ye, Bang-Ce
AU  - Ye BC
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Mol Biotechnol
JT  - Molecular biotechnology
JID - 9423533
RN  - 0 (DNA Probes)
RN  - 0 (HLA-DQ Antigens)
RN  - 0 (Oligonucleotides)
SB  - IM
MH  - DNA Probes/*chemistry
MH  - Genotype
MH  - HLA-DQ Antigens/*genetics
MH  - Humans
MH  - Oligonucleotide Array Sequence Analysis/*methods
MH  - Oligonucleotides/*chemistry
EDAT- 2007/10/05 09:00
MHDA- 2007/11/10 09:00
CRDT- 2007/10/05 09:00
PHST- 1999/11/30 00:00 [received]
PHST- 1999/11/30 00:00 [revised]
PHST- 1999/11/30 00:00 [accepted]
PHST- 2007/10/05 09:00 [pubmed]
PHST- 2007/11/10 09:00 [medline]
PHST- 2007/10/05 09:00 [entrez]
AID - MB:36:2:142 [pii]
AID - 10.1007/s12033-007-0011-7 [doi]
PST - ppublish
SO  - Mol Biotechnol. 2007 Jun;36(2):142-50. doi: 10.1007/s12033-007-0011-7.