PMID- 17914874
OWN - NLM
STAT- MEDLINE
DCOM- 20071227
LR  - 20220318
IS  - 0021-8561 (Print)
IS  - 0021-8561 (Linking)
VI  - 55
IP  - 22
DP  - 2007 Oct 31
TI  - Simple multiresidue method for monitoring of trimethoprim and sulfonamide 
      residues in buffalo meat by high-performance liquid chromatography.
PG  - 8845-50
AB  - A simple, specific, and rapid analytical method for the determination of 
      trimethoprim (TMP) and three sulfonamide (SA) antimicrobial drug residues in 
      buffalo meat is developed and validated. This method is based on a solid-phase 
      extraction technique followed by high-performance liquid chromatography 
      (HPLC)-photodiode array (PDA) detection. Target compounds were extracted from the 
      meat by acetonitrile and water, cleaned up on a Bond Elute C 18 cartridge column, 
      and separated on a RP-C 18 column during HPLC analysis. Acetonitrile along with 
      water appears to be an excellent extractant as recovery of the analytes at 
      maximum residues levels (MRLs) in spiked sample was in the range of 75-108%, with 
      coefficient of variations (CVs) ranging between 1.34 and 22%. The limit of 
      detection (LOD) and the limit of quantification (LOQ) were 0.031 and 0.062 
      microg/g, respectively, for all of the compounds. Intra- and interday assay 
      precisions of the method at 0.125 microg/g concentrations for any drug ranged 
      between 3 and 4%. The linearities of the TMP, sulfadimidine (SDM), sulfadoxine 
      (SDO), and sulfamethoxazole (SMX) were 0.9989, 0.9999, 0.9998, and 0.9997, 
      respectively. For robustness, the analytical method was applied to 122 buffalo 
      meat samples obtained from export meat processing plants.
FAU - Biswas, A K
AU  - Biswas AK
AD  - Division of Livestock Products Technology, Indian Veterinary Research Institute, 
      Izatnagar 243122 (UP), India. biswaslpt@gmail.com
FAU - Rao, G S
AU  - Rao GS
FAU - Kondaiah, N
AU  - Kondaiah N
FAU - Anjaneyulu, A S R
AU  - Anjaneyulu AS
FAU - Malik, J K
AU  - Malik JK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20071004
PL  - United States
TA  - J Agric Food Chem
JT  - Journal of agricultural and food chemistry
JID - 0374755
RN  - 0 (Anti-Infective Agents)
RN  - 0 (Sulfonamides)
RN  - AN164J8Y0X (Trimethoprim)
SB  - IM
MH  - Anti-Infective Agents/*analysis
MH  - Chromatography, High Pressure Liquid
MH  - Drug Residues
MH  - Reproducibility of Results
MH  - Sulfonamides/*analysis
MH  - Trimethoprim/*analysis
EDAT- 2007/10/05 09:00
MHDA- 2007/12/28 09:00
CRDT- 2007/10/05 09:00
PHST- 2007/10/05 09:00 [pubmed]
PHST- 2007/12/28 09:00 [medline]
PHST- 2007/10/05 09:00 [entrez]
AID - 10.1021/jf071140w [doi]
PST - ppublish
SO  - J Agric Food Chem. 2007 Oct 31;55(22):8845-50. doi: 10.1021/jf071140w. Epub 2007 
      Oct 4.