PMID- 17914946
OWN - NLM
STAT- MEDLINE
DCOM- 20080118
LR  - 20091119
IS  - 0815-9319 (Print)
IS  - 0815-9319 (Linking)
VI  - 22
IP  - 11
DP  - 2007 Nov
TI  - Gastrointestinal stromal tumors: usefulness of immunohistochemistry, flow 
      cytometry and fluorescence in situ hybridization.
PG  - 1754-9
AB  - BACKGROUND AND AIMS: Gastrointestinal stromal tumors (GIST) constitute a group of 
      primary mesenchymal tumors of the gastrointestinal tract, known for their 
      diversity in clinical behavior and the difficulties in determining malignancy and 
      prognosis. This retrospective study evaluated a series of GIST by means of 
      immunohistochemical techniques, flow cytometry and fluorescence in situ 
      hybridization (FISH). METHODS: Nine patients with GIST were analyzed for tumor 
      size, mitotic count and CD117, CD34, MIB-1 with immunohistochemistry. In 
      addition, the GIST were tested with FISH for chromosomes 8 and 17 and DNA index 
      was evaluated by flow cytometry. RESULTS: The findings confirmed the usefulness 
      of CD117 and CD34 in diagnosing GIST and the prognostic role of MIB-1, but do not 
      support a correlation between aneuploidy in flow cytometry and poor outcome. The 
      FISH results suggest close follow-up for patients with benign GIST with a 
      numerical alteration of chromosome 8. The technique could select patients with 
      tumors at high-risk with aneusomy of chromosome 17. CONCLUSION: This study shows 
      the possible application of FISH to the evaluation of patients with GIST, in 
      addition to analysis of morphological features.
FAU - Fontana, Maria Grazia
AU  - Fontana MG
AD  - First Department of Surgery, University of Brescia, Brescia, Italy.
FAU - Rossi, Elisa
AU  - Rossi E
FAU - Bassotti, Gabrio
AU  - Bassotti G
FAU - Aquilano, Maria Costanza
AU  - Aquilano MC
FAU - Cadei, Morris
AU  - Cadei M
FAU - Grigolato, Piergiovanni
AU  - Grigolato P
FAU - Villanacci, Vincenzo
AU  - Villanacci V
LA  - eng
PT  - Journal Article
PL  - Australia
TA  - J Gastroenterol Hepatol
JT  - Journal of gastroenterology and hepatology
JID - 8607909
RN  - 0 (Antigens, CD34)
RN  - 0 (Ki-67 Antigen)
RN  - EC 2.7.10.1 (Proto-Oncogene Proteins c-kit)
SB  - IM
MH  - Antigens, CD34/analysis
MH  - Chromosomes, Human, Pair 17
MH  - Chromosomes, Human, Pair 8
MH  - Female
MH  - *Flow Cytometry
MH  - Follow-Up Studies
MH  - Gastrointestinal Stromal Tumors/*diagnosis/genetics/pathology/therapy
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - *Immunohistochemistry
MH  - *In Situ Hybridization, Fluorescence
MH  - Ki-67 Antigen/analysis
MH  - Male
MH  - Middle Aged
MH  - Mitotic Index
MH  - Neoplasm Staging
MH  - Predictive Value of Tests
MH  - Prognosis
MH  - Proto-Oncogene Proteins c-kit/analysis
MH  - Retrospective Studies
MH  - Time Factors
EDAT- 2007/10/05 09:00
MHDA- 2008/01/19 09:00
CRDT- 2007/10/05 09:00
PHST- 2007/10/05 09:00 [pubmed]
PHST- 2008/01/19 09:00 [medline]
PHST- 2007/10/05 09:00 [entrez]
AID - JGH4530 [pii]
AID - 10.1111/j.1440-1746.2006.04530.x [doi]
PST - ppublish
SO  - J Gastroenterol Hepatol. 2007 Nov;22(11):1754-9. doi: 
      10.1111/j.1440-1746.2006.04530.x.