PMID- 17915231
OWN - NLM
STAT- MEDLINE
DCOM- 20080226
LR  - 20191210
IS  - 0021-9673 (Print)
IS  - 0021-9673 (Linking)
VI  - 1170
IP  - 1-2
DP  - 2007 Nov 2
TI  - Determination of betaine, choline and trimethylamine in feed additive by 
      ion-exchange liquid chromatography/non-suppressed conductivity detection.
PG  - 114-7
AB  - An ion chromatography method with non-suppressed conductivity detection was 
      developed for the simultaneous determination of betaine, choline and 
      trimethylamine in feed additive. The analytes and the common inorganic cations 
      were well separated by means of cation-exchange chromatography using a 4.5 mmol/L 
      methanesulfonic acid solution containing 10% (v/v) acetonitrile as eluent and an 
      IonPac SCS1 column (250 mm x 4 mm i.d.) as the separation column. The effects of 
      the different chromatographic parameters on the separation were also 
      investigated. Detection limits of betaine, choline and trimethylamine were 0.076, 
      0.044 and 0.041 mg/L. The relative standard deviations (RSDs) of the retention 
      time and peak area were less than 0.30% and 0.88%, respectively. The recoveries 
      were between 93.2% and 112.6%. The method is suitable for use as a routine method 
      in production quality control of feed additive.
FAU - Zhang, Jiajie
AU  - Zhang J
AD  - Department of Chemistry, Zhangjiang University, Hangzhou 310028, China.
FAU - Zhu, Yan
AU  - Zhu Y
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070914
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 0 (Cation Exchange Resins)
RN  - 0 (Cations)
RN  - 0 (Methylamines)
RN  - 3SCV180C9W (Betaine)
RN  - LHH7G8O305 (trimethylamine)
RN  - N91BDP6H0X (Choline)
SB  - IM
MH  - Animal Feed/*analysis
MH  - Betaine/*analysis
MH  - Cation Exchange Resins/chemistry
MH  - Cations/chemistry
MH  - Choline/*analysis
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Chromatography, Ion Exchange/*methods
MH  - *Electric Conductivity
MH  - Methylamines/*analysis
MH  - Reference Standards
MH  - Reproducibility of Results
EDAT- 2007/10/05 09:00
MHDA- 2008/02/27 09:00
CRDT- 2007/10/05 09:00
PHST- 2007/06/21 00:00 [received]
PHST- 2007/09/01 00:00 [revised]
PHST- 2007/09/11 00:00 [accepted]
PHST- 2007/10/05 09:00 [pubmed]
PHST- 2008/02/27 09:00 [medline]
PHST- 2007/10/05 09:00 [entrez]
AID - S0021-9673(07)01552-X [pii]
AID - 10.1016/j.chroma.2007.09.014 [doi]
PST - ppublish
SO  - J Chromatogr A. 2007 Nov 2;1170(1-2):114-7. doi: 10.1016/j.chroma.2007.09.014. 
      Epub 2007 Sep 14.