PMID- 17915260
OWN - NLM
STAT- MEDLINE
DCOM- 20071126
LR  - 20211020
IS  - 0024-3205 (Print)
IS  - 0024-3205 (Linking)
VI  - 81
IP  - 16
DP  - 2007 Sep 29
TI  - Nitric oxide signaling participates in norepinephrine-induced activity of 
      neuronal intracellular survival pathways.
PG  - 1280-90
AB  - Much evidence has gathered that nitric oxide (NO) signaling, via cGMP-dependent 
      mechanisms, may activate pro-survival pathways in hippocampal neurons and inhibit 
      apoptosis. Past research has revealed that the enhancement of monoaminergic 
      neurotransmission via exercise or treatment with antidepressant medications leads 
      to an enhanced expression of brain-derived neurotrophic factor (BDNF). In 
      isolated hippocampal neurons, norepinephrine (NE) application also increases the 
      immunoreactivity of BDNF and several pro-survival signaling molecules. The data 
      herein support the possibility that NO signaling plays an important role in 
      enhancing neurotrophin expression and activation of the pro-survival 
      phosphatidylinositol 3' kinase (PI-3K) pathway stimulated by NE. In isolated 
      hippocampal neurons, the NO donor, sodium nitroprusside, increases BDNF, PI-3K, 
      and phospho-ERK1 immunoreactivity. Specific inhibitors of the NO system suggest 
      that NE-induced increases in hippocampal BDNF and the PI-3K pathway, but not 
      stimulation of the MAPK pathway, depend upon NO signaling. In addition, 
      inhibiting cGMP suggest that the effects of NE on BDNF immunoreactivity and Akt 
      phosphorylation are also cGMP-dependent. Finally, the application of l-NAME to 
      hippocampal neurons increases cell death. This is the first study of its kind 
      demonstrating the involvement of NE-induced pro-survival signaling in three 
      distinct signaling pathways: PI-3K, MAPK, and NO/cGMP. Possible mechanisms are 
      discussed in light of the results.
FAU - Chen, Michael J
AU  - Chen MJ
AD  - Department of Biological Sciences, California State University, 5151 State 
      University Drive, Los Angeles, CA 90032, USA. mjchen@calstatela.edu
FAU - Russo-Neustadt, Amelia A
AU  - Russo-Neustadt AA
LA  - eng
GR  - R01 MH059776/MH/NIMH NIH HHS/United States
GR  - R01 MH059776-10/MH/NIMH NIH HHS/United States
GR  - MH 59776/MH/NIMH NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20070915
PL  - Netherlands
TA  - Life Sci
JT  - Life sciences
JID - 0375521
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Nitric Oxide Donors)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase)
RN  - EC 2.7.1.- (Phosphatidylinositol 3-Kinases)
RN  - EC 2.7.12.2 (Mitogen-Activated Protein Kinase Kinases)
RN  - EC 4.6.1.2 (Guanylate Cyclase)
RN  - H2D2X058MU (Cyclic GMP)
RN  - X4W3ENH1CV (Norepinephrine)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Brain-Derived Neurotrophic Factor/metabolism
MH  - Cell Survival/drug effects
MH  - Cells, Cultured
MH  - Cyclic GMP/metabolism
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Enzyme Inhibitors/pharmacology
MH  - Female
MH  - Gestational Age
MH  - Guanylate Cyclase/antagonists & inhibitors
MH  - *Hippocampus/cytology/embryology/metabolism
MH  - Mitogen-Activated Protein Kinase Kinases/metabolism
MH  - *Neurons/cytology/drug effects/metabolism
MH  - Nitric Oxide/*metabolism
MH  - Nitric Oxide Donors/pharmacology
MH  - Nitric Oxide Synthase/antagonists & inhibitors
MH  - Norepinephrine/*pharmacology
MH  - Phosphatidylinositol 3-Kinases/metabolism
MH  - Rats
MH  - Signal Transduction
PMC - PMC2435382
MID - NIHMS32991
EDAT- 2007/10/05 09:00
MHDA- 2007/12/06 09:00
PMCR- 2008/06/23
CRDT- 2007/10/05 09:00
PHST- 2007/06/27 00:00 [received]
PHST- 2007/08/08 00:00 [revised]
PHST- 2007/09/05 00:00 [accepted]
PHST- 2007/10/05 09:00 [pubmed]
PHST- 2007/12/06 09:00 [medline]
PHST- 2007/10/05 09:00 [entrez]
PHST- 2008/06/23 00:00 [pmc-release]
AID - S0024-3205(07)00664-9 [pii]
AID - 10.1016/j.lfs.2007.09.003 [doi]
PST - ppublish
SO  - Life Sci. 2007 Sep 29;81(16):1280-90. doi: 10.1016/j.lfs.2007.09.003. Epub 2007 
      Sep 15.