PMID- 17916946
OWN - NLM
STAT- MEDLINE
DCOM- 20080110
LR  - 20150813
IS  - 0163-4984 (Print)
IS  - 0163-4984 (Linking)
VI  - 119
IP  - 3
DP  - 2007 Dec
TI  - New developments in selenium biochemistry: selenocysteine biosynthesis in 
      eukaryotes and archaea.
PG  - 234-41
AB  - We used comparative genomics and experimental analyses to show that (1) 
      eukaryotes and archaea, which possess the selenocysteine (Sec) protein insertion 
      machinery contain an enzyme, O-phosphoseryl-transfer RNA (tRNA) [Ser]Sec kinase 
      (designated PSTK), which phosphorylates seryl-tRNA [Ser]Sec to form 
      O-phosphoseryl-tRNA [Ser]Sec and (2) the Sec synthase (SecS) in mammals is a 
      pyridoxal phosphate-containing protein previously described as the soluble liver 
      antigen (SLA). SecS uses the product of PSTK, O-phosphoseryl-tRNA[Ser]Sec, and 
      selenophosphate as substrates to generate selenocysteyl-tRNA [Ser]Sec. Sec could 
      be synthesized on tRNA [Ser]Sec from selenide, adenosine triphosphate (ATP), and 
      serine using tRNA[Ser]Sec, seryl-tRNA synthetase, PSTK, selenophosphate 
      synthetase, and SecS. The enzyme that synthesizes monoselenophosphate is a 
      previously identified selenoprotein, selenophosphate synthetase 2 (SPS2), whereas 
      the previously identified mammalian selenophosphate synthetase 1 did not serve 
      this function. Monoselenophosphate also served directly in the reaction replacing 
      ATP, selenide, and SPS2, demonstrating that this compound was the active selenium 
      donor. Conservation of the overall pathway of Sec biosynthesis suggests that this 
      pathway is also active in other eukaryotes and archaea that contain 
      selenoproteins.
FAU - Xu, Xue-Ming
AU  - Xu XM
AD  - Molecular Biology of Selenium Section, Laboratory of Cancer Prevention, Center 
      for Cancer Research, National Institutes of Health, Bethesda, MD 20892, USA.
FAU - Carlson, Bradley A
AU  - Carlson BA
FAU - Zhang, Yan
AU  - Zhang Y
FAU - Mix, Heiko
AU  - Mix H
FAU - Kryukov, Gregory V
AU  - Kryukov GV
FAU - Glass, Richard S
AU  - Glass RS
FAU - Berry, Marla J
AU  - Berry MJ
FAU - Gladyshev, Vadim N
AU  - Gladyshev VN
FAU - Hatfield, Dolph L
AU  - Hatfield DL
LA  - eng
GR  - CA-41108/CA/NCI NIH HHS/United States
GR  - CA080946/CA/NCI NIH HHS/United States
GR  - DK47320/DK/NIDDK NIH HHS/United States
GR  - DK52963/DK/NIDDK NIH HHS/United States
GR  - GM061603/GM/NIGMS NIH HHS/United States
GR  - GM065204/GM/NIGMS NIH HHS/United States
GR  - Intramural NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, N.I.H., Intramural
PL  - United States
TA  - Biol Trace Elem Res
JT  - Biological trace element research
JID - 7911509
RN  - 0 (RNA, Transfer, Amino Acyl)
RN  - 0 (Selenoproteins)
RN  - 0CH9049VIS (Selenocysteine)
RN  - EC 2.7.- (Phosphotransferases)
RN  - EC 2.7.9.3 (selenophosphate synthetase)
RN  - H6241UJ22B (Selenium)
SB  - IM
MH  - Animals
MH  - Archaea/*metabolism
MH  - Humans
MH  - Mammals/metabolism
MH  - Models, Biological
MH  - Phosphotransferases/metabolism
MH  - RNA, Transfer, Amino Acyl/metabolism
MH  - Selenium/*metabolism
MH  - Selenocysteine/*biosynthesis
MH  - Selenoproteins/metabolism
EDAT- 2007/10/06 09:00
MHDA- 2008/01/11 09:00
CRDT- 2007/10/06 09:00
PHST- 1999/11/30 00:00 [received]
PHST- 1999/11/30 00:00 [revised]
PHST- 1999/11/30 00:00 [accepted]
PHST- 2007/10/06 09:00 [pubmed]
PHST- 2008/01/11 09:00 [medline]
PHST- 2007/10/06 09:00 [entrez]
AID - BTER:119:3:234 [pii]
AID - 10.1007/s12011-007-8003-9 [doi]
PST - ppublish
SO  - Biol Trace Elem Res. 2007 Dec;119(3):234-41. doi: 10.1007/s12011-007-8003-9.