PMID- 17917571
OWN - NLM
STAT- MEDLINE
DCOM- 20071221
LR  - 20221207
IS  - 1089-2591 (Print)
IS  - 1089-2591 (Linking)
VI  - 11
IP  - 4
DP  - 2007 Oct
TI  - Premalignant cervical lesions are characterized by dihydrofolate reductase gene 
      amplification and c-Myc overexpression: possible biomarkers.
PG  - 265-72
AB  - OBJECTIVE: The c-Myc oncoprotein deregulation is associated with overall genomic 
      instability and locus-specific genomic instability involving the dihydrofolate 
      reductase (DHFR) locus. This study analyzes c-Myc protein levels and the 
      stability of the DHFR gene in cervical tissue biopsies. MATERIALS AND METHODS: 
      The stability of the DHFR gene was examined by fluorescence in situ hybridization 
      (FISH). c-Myc protein levels were evaluated using quantitative fluorescent 
      immunohistochemistry. Forty-four cervical tissue biopsies were analyzed and 
      included 33 preinvasive cervical lesions identified by histology, 14 samples were 
      cervical intraepithelial neoplasia (CIN) 1; 7 were CIN 2; and 12 were CIN 3. 
      Eleven biopsies had negative histology. RESULTS AND CONCLUSION: c-Myc protein 
      levels were elevated in CIN 1, 2, and 3 (p = .02) biopsies. Concomitantly, DHFR 
      gene amplification was detected in CIN 1, 2, and 3 (p = .0001). The degrees of 
      DHFR gene amplification and of c-Myc protein levels were a measure of the 
      progressive degree of the lesion.
FAU - Guijon, Fernando B
AU  - Guijon FB
AD  - Department of Obstetrics, Gynecology and Reproductive Sciences, University of 
      Manitoba, Winnipeg, Manitoba, Canada. guijon@cc.umanitoba.ca
FAU - Greulich-Bode, Km
AU  - Greulich-Bode K
FAU - Paraskevas, Maria
AU  - Paraskevas M
FAU - Baker, Patricia
AU  - Baker P
FAU - Mai, Sabine
AU  - Mai S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Low Genit Tract Dis
JT  - Journal of lower genital tract disease
JID - 9704963
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (MYC protein, human)
RN  - 0 (Proto-Oncogene Proteins c-myc)
RN  - EC 1.5.1.3 (Tetrahydrofolate Dehydrogenase)
SB  - IM
MH  - Biomarkers, Tumor
MH  - Colposcopy
MH  - Cytogenetic Analysis
MH  - Female
MH  - Frozen Sections
MH  - Gene Expression
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Proto-Oncogene Proteins c-myc/*metabolism
MH  - Tetrahydrofolate Dehydrogenase/*genetics
MH  - Uterine Cervical Neoplasms/*genetics
MH  - Uterine Cervical Dysplasia/*genetics
EDAT- 2007/10/06 09:00
MHDA- 2007/12/22 09:00
CRDT- 2007/10/06 09:00
PHST- 2007/10/06 09:00 [pubmed]
PHST- 2007/12/22 09:00 [medline]
PHST- 2007/10/06 09:00 [entrez]
AID - 00128360-200710000-00006 [pii]
AID - 10.1097/LGT.0b013e31803c4df0 [doi]
PST - ppublish
SO  - J Low Genit Tract Dis. 2007 Oct;11(4):265-72. doi: 10.1097/LGT.0b013e31803c4df0.