PMID- 17917697
OWN - NLM
STAT- MEDLINE
DCOM- 20080109
LR  - 20220409
IS  - 1434-5161 (Print)
IS  - 1434-5161 (Linking)
VI  - 52
IP  - 11
DP  - 2007
TI  - Suggestive evidence for chromosomal localization of non-coding RNA from imprinted 
      LIT1.
PG  - 926-933
LID - 10.1007/s10038-007-0196-4 [doi]
AB  - The non-coding RNA LIT1/KCNQ1OT1, itself the product of an imprinted gene, is 
      involved in cis-limited silencing within an imprinted cluster on human chromosome 
      11p15.5. Although the locus serves as an imprinting center, the mechanism of 
      transcriptional regulation is not clear. To help understand the function of the 
      LIT1 non-coding RNA, we used fluorescence in situ hybridization (FISH) to examine 
      the sub-cellular localization of LIT1 RNA molecules. LIT1 RNA signals were 
      observed in most of the interphase human lymphoblast and fibroblast cells. The 
      RNA also appeared to accumulate on neighboring regions of chromatin containing 
      the SLC22A18/IMPT1 and CDKN1C/p57KIP2 genes, as shown by high-resolution fiber 
      RNA FISH and modified RNA TRAP (tagging and recovery of associated proteins) 
      methods. These results suggest that LIT1 RNA stably localizes to a specific 
      chromatin region and plays an important role in the transcriptional silencing of 
      the imprinting domain.
FAU - Murakami, Kazuhiro
AU  - Murakami K
AD  - Department of Biomedical Science, Graduate School of Medical Science, Tottori 
      University, 86 Nishicho, Yonago, 683-8503, Japan.
FAU - Oshimura, Mitsuo
AU  - Oshimura M
AD  - Department of Biomedical Science, Graduate School of Medical Science, Tottori 
      University, 86 Nishicho, Yonago, 683-8503, Japan.
FAU - Kugoh, Hiroyuki
AU  - Kugoh H
AD  - Department of Biomedical Science, Graduate School of Medical Science, Tottori 
      University, 86 Nishicho, Yonago, 683-8503, Japan. 
      kugoh@grape.med.tottori-u.ac.jp.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20071005
PL  - England
TA  - J Hum Genet
JT  - Journal of human genetics
JID - 9808008
RN  - 0 (KCNQ1OT1 RNA)
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (RNA, Untranslated)
SB  - IM
MH  - *Chromosome Mapping
MH  - *Genomic Imprinting
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - RNA, Long Noncoding
MH  - RNA, Untranslated/*genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 2007/10/06 09:00
MHDA- 2008/01/10 09:00
CRDT- 2007/10/06 09:00
PHST- 2007/06/01 00:00 [received]
PHST- 2007/09/05 00:00 [accepted]
PHST- 2007/10/06 09:00 [pubmed]
PHST- 2008/01/10 09:00 [medline]
PHST- 2007/10/06 09:00 [entrez]
AID - 10.1007/s10038-007-0196-4 [pii]
AID - 10.1007/s10038-007-0196-4 [doi]
PST - ppublish
SO  - J Hum Genet. 2007;52(11):926-933. doi: 10.1007/s10038-007-0196-4. Epub 2007 Oct 
      5.