PMID- 17919519
OWN - NLM
STAT- MEDLINE
DCOM- 20080102
LR  - 20131121
IS  - 0009-3084 (Print)
IS  - 0009-3084 (Linking)
VI  - 150
IP  - 2
DP  - 2007 Dec
TI  - Apoptosis-associated changes in the glycerophospholipid composition of 
      hematopoietic progenitor cells monitored by 31P NMR spectroscopy and MALDI-TOF 
      mass spectrometry.
PG  - 229-38
AB  - Apoptosis, or programmed cell death, plays an important role in development and 
      in tissue homeostasis and is assumed to be accompanied by changes in the 
      composition of cellular glycerophospholipids (GPL). We have applied a combination 
      of 31P nuclear magnetic resonance spectroscopy and matrix-assisted laser 
      desorption and ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) to 
      the analysis of organic extracts of hematopoietic progenitor cells undergoing the 
      physiologically relevant process of apoptosis following growth factor withdrawal. 
      The combined application of these methods enables the quantitative analysis of 
      all glycerophospholipid classes and reveals changes in the acyl chain 
      compositions from crude cell extracts. Using these techniques, an increase in the 
      ratio of ether-linked glycerophospholipids to diacyl-glycerophospholipids during 
      apoptosis was observed. The relative decrease in the membrane 
      diacyl-phosphatidylcholine (PC) levels was found to correlate with increased 
      concentrations of the corresponding lysophosphatidylcholine (LPC) present in the 
      medium.
FAU - Fuchs, Beate
AU  - Fuchs B
AD  - University of Leipzig, Medical Faculty, Institute of Medical Physics and 
      Biophysics, Hartelstr. 16-18, D-04107 Leipzig, Germany. 
      Beate.Fuchs@medizin.uni-leipzig.de
FAU - Schiller, Jurgen
AU  - Schiller J
FAU - Cross, Michael A
AU  - Cross MA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070901
PL  - Ireland
TA  - Chem Phys Lipids
JT  - Chemistry and physics of lipids
JID - 0067206
RN  - 0 (Ethanolamines)
RN  - 0 (Glycerophospholipids)
RN  - 0 (Interleukin-3)
RN  - 0 (Lipids)
RN  - 0 (Phospholipids)
RN  - 107-73-3 (Phosphorylcholine)
RN  - PDC6A3C0OX (Glycerol)
SB  - IM
MH  - *Apoptosis
MH  - Biochemistry/*methods
MH  - Ethanolamines/chemistry
MH  - Glycerol/chemistry
MH  - Glycerophospholipids/*metabolism
MH  - Hematopoietic Stem Cells/*metabolism
MH  - Humans
MH  - Interleukin-3/metabolism
MH  - Lipids/chemistry
MH  - Magnetic Resonance Spectroscopy/*methods
MH  - Mass Spectrometry/*methods
MH  - Phospholipids/chemistry
MH  - Phosphorylcholine/chemistry
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/*methods
EDAT- 2007/10/09 09:00
MHDA- 2008/01/03 09:00
CRDT- 2007/10/09 09:00
PHST- 2007/03/21 00:00 [received]
PHST- 2007/08/27 00:00 [revised]
PHST- 2007/08/28 00:00 [accepted]
PHST- 2007/10/09 09:00 [pubmed]
PHST- 2008/01/03 09:00 [medline]
PHST- 2007/10/09 09:00 [entrez]
AID - S0009-3084(07)00421-5 [pii]
AID - 10.1016/j.chemphyslip.2007.08.005 [doi]
PST - ppublish
SO  - Chem Phys Lipids. 2007 Dec;150(2):229-38. doi: 10.1016/j.chemphyslip.2007.08.005. 
      Epub 2007 Sep 1.