PMID- 17919921 OWN - NLM STAT- MEDLINE DCOM- 20080404 LR - 20131121 IS - 1044-7431 (Print) IS - 1044-7431 (Linking) VI - 37 IP - 1 DP - 2008 Jan TI - BDNF mRNA splice variants display activity-dependent targeting to distinct hippocampal laminae. PG - 11-9 AB - Brain-derived neurotrophic factor (BDNF) may exert contrasting effects depending on its different subcellular sites of action (soma, dendrites, axons). These contrasting effects may explain contradictory findings, for example that BDNF may favour or oppose epileptogenesis. We determined the distribution of five BDNF splice variants in the soma and dendrites of rat hippocampal principal neurons, after application of stimuli that prompt BDNF mRNA accumulation in dendrites (epileptogenic seizures). Under basal conditions, no BDNF mRNA splice variant was detectable in dendrites, while specific splice variants were found in dendrites in response to epileptogenic seizures. Three hours after pilocarpine administration, exon VI and exon II splice variants were found in dendrites, while exons I and IV transcripts displayed a strictly somatic localization. Three hours after kainate administration, only exon VI was found in dendrites. These data suggest that the regulated expression of different splice variants may provide a spatial code to ensure the delivery of BDNF to precise destinations in the cell soma or along the dendrites. FAU - Chiaruttini, C AU - Chiaruttini C AD - BRAIN Centre for Neuroscience, Department of Biology, University of Trieste, 34127 Trieste, Italy. FAU - Sonego, M AU - Sonego M FAU - Baj, G AU - Baj G FAU - Simonato, M AU - Simonato M FAU - Tongiorgi, E AU - Tongiorgi E LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20070823 PL - United States TA - Mol Cell Neurosci JT - Molecular and cellular neurosciences JID - 9100095 RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (RNA, Messenger) RN - 01MI4Q9DI3 (Pilocarpine) RN - SIV03811UC (Kainic Acid) SB - IM MH - Alternative Splicing/*physiology MH - Analysis of Variance MH - Animals MH - Brain-Derived Neurotrophic Factor/*genetics/metabolism MH - Dendrites MH - Epilepsy/chemically induced/pathology MH - Exons/physiology MH - Gene Expression Regulation/drug effects/physiology MH - Hippocampus/*cytology MH - In Situ Hybridization MH - Kainic Acid MH - Male MH - Neurons/*cytology/*metabolism MH - Pilocarpine MH - RNA, Messenger/*metabolism MH - Rats MH - Rats, Sprague-Dawley EDAT- 2007/10/09 09:00 MHDA- 2008/04/05 09:00 CRDT- 2007/10/09 09:00 PHST- 2007/03/19 00:00 [received] PHST- 2007/08/06 00:00 [revised] PHST- 2007/08/16 00:00 [accepted] PHST- 2007/10/09 09:00 [pubmed] PHST- 2008/04/05 09:00 [medline] PHST- 2007/10/09 09:00 [entrez] AID - S1044-7431(07)00197-2 [pii] AID - 10.1016/j.mcn.2007.08.011 [doi] PST - ppublish SO - Mol Cell Neurosci. 2008 Jan;37(1):11-9. doi: 10.1016/j.mcn.2007.08.011. Epub 2007 Aug 23.