PMID- 17922181
OWN - NLM
STAT- MEDLINE
DCOM- 20080129
LR  - 20240328
IS  - 0920-8569 (Print)
IS  - 1572-994X (Electronic)
IS  - 0920-8569 (Linking)
VI  - 35
IP  - 3
DP  - 2007 Dec
TI  - Co-expressing GP5 and M proteins under different promoters in recombinant 
      modified vaccinia virus ankara (rMVA)-based vaccine vector enhanced the humoral 
      and cellular immune responses of porcine reproductive and respiratory syndrome 
      virus (PRRSV).
PG  - 585-95
AB  - The porcine reproductive and respiratory syndrome virus (PRRSV) has three major 
      structural proteins which designated as GP5, M, and N. Protein GP5 and M have 
      been considered very important to arouse the humoral and cellular immune 
      responses against PRRSV infection and proposed to be the excellent candidate 
      proteins in the design of PRRS bioengineering vaccine. There were some attempts 
      on expressing GP5 or M in DNA vaccine and adenovirus to arouse humoral and 
      cellular immune responses, but few papers have been reported on that the immune 
      response can be difference because of the expression patterns of GP5 and M 
      proteins in the recombinant virus. In this article, four recombinant viruses that 
      expressed GP5 and M proteins of PRRSV in the modified vaccinia virus ankara (MVA) 
      with different expression patterns were made. In these recombinant virus (rMVAs), 
      GP5 and M proteins were expressed in MVA in the same virus but under the control 
      of two promoters (rMVA-GP5/M), or as a fusion protein under one promoter 
      (rMVA-GP5-M), or separately (rMVA-GP5 and rMVA-M). The humoral and cellular 
      immune responses for the four recombinant viruses were evaluated with mouse 
      model. Every mouse was inoculated with 5 x 10(5) TCID50 of the different rMVAs 
      and boosted 3 weeks later. Neutralizing antibody titers for each group were 
      detected with virus neutralization test assay weekly after the primary 
      inoculation for 13 weeks to evaluate the humoral immune response. The production 
      of gamma interferon (IFN-gamma), interleukin-2 (IL-2), and interleukin-4 (IL-4) 
      was detected in splenocytes of rMVA-inoculated mice at 30, 60, and 90 days post 
      inoculation to evaluate the cellular immune response. Results showed that 
      rMVA-GP5 and rMVA-M cannot induce obvious humoral and cellular immune responses; 
      rMVA-GP5-M inoculated group developed better immune responses than rMVA-GP5 and 
      rMVA-M inoculated groups; however, mice inoculated with rMVA-GP5/M maintained the 
      strongest cellular response against PRRS and consistently enhanced the anti-PRRSV 
      humoral responses. The strategy of co-expressing PRRSV GP5 and M protein in MVA 
      under the control of different promoters might be an attractive method for future 
      PRRSV vaccine design.
FAU - Zheng, Qisheng
AU  - Zheng Q
AD  - Key Laboratory of Animal Disease Diagnosis and Immunology, College of Veterinary 
      Medicine, Nanjing Agricultural University, Nanjing 210095, PR China. 
      zhengqisheng2004@yahoo.com.cn
FAU - Chen, Desheng
AU  - Chen D
FAU - Li, Peng
AU  - Li P
FAU - Bi, Zhixiang
AU  - Bi Z
FAU - Cao, Ruibing
AU  - Cao R
FAU - Zhou, Bin
AU  - Zhou B
FAU - Chen, Puyan
AU  - Chen P
LA  - eng
PT  - Journal Article
DEP - 20071006
PL  - United States
TA  - Virus Genes
JT  - Virus genes
JID - 8803967
RN  - 0 (Antibodies, Viral)
RN  - 0 (Interleukin-2)
RN  - 0 (M protein, porcine reproductive and respiratory syndrome virus)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Vaccines, Synthetic)
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (Viral Matrix Proteins)
RN  - 0 (Viral Vaccines)
RN  - 0 (glycoprotein 5, PRRSV)
RN  - 207137-56-2 (Interleukin-4)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/blood
MH  - *Genetic Vectors
MH  - Immunization, Secondary
MH  - Interferon-gamma/biosynthesis
MH  - Interleukin-2/biosynthesis
MH  - Interleukin-4/biosynthesis
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Neutralization Tests
MH  - Porcine Reproductive and Respiratory Syndrome/*immunology
MH  - Porcine respiratory and reproductive syndrome virus/genetics/*immunology
MH  - Promoter Regions, Genetic
MH  - Recombinant Fusion Proteins/biosynthesis/genetics/immunology
MH  - Recombinant Proteins/biosynthesis/genetics/immunology
MH  - Spleen/immunology
MH  - T-Lymphocytes/immunology
MH  - Vaccines, Synthetic/immunology
MH  - Vaccinia virus/*genetics
MH  - Viral Envelope Proteins/genetics/*immunology
MH  - Viral Matrix Proteins/genetics/*immunology
MH  - Viral Vaccines/*immunology
PMC - PMC7088781
EDAT- 2007/10/09 09:00
MHDA- 2008/01/30 09:00
PMCR- 2020/03/23
CRDT- 2007/10/09 09:00
PHST- 2007/04/30 00:00 [received]
PHST- 2007/08/22 00:00 [accepted]
PHST- 2007/10/09 09:00 [pubmed]
PHST- 2008/01/30 09:00 [medline]
PHST- 2007/10/09 09:00 [entrez]
PHST- 2020/03/23 00:00 [pmc-release]
AID - 161 [pii]
AID - 10.1007/s11262-007-0161-5 [doi]
PST - ppublish
SO  - Virus Genes. 2007 Dec;35(3):585-95. doi: 10.1007/s11262-007-0161-5. Epub 2007 Oct 
      6.