PMID- 17922833
OWN - NLM
STAT- MEDLINE
DCOM- 20080327
LR  - 20221109
IS  - 1365-2567 (Electronic)
IS  - 0019-2805 (Print)
IS  - 0019-2805 (Linking)
VI  - 123
IP  - 3
DP  - 2008 Mar
TI  - Neuropeptides activate human mast cell degranulation and chemokine production.
PG  - 398-410
AB  - During neuronal-induced inflammation, mast cells may respond to stimuli such as 
      neuropeptides in an FcepsilonRI-independent manner. In this study, we 
      characterized human mast cell responses to substance P (SP), nerve growth factor 
      (NGF), calcitonin gene-related peptide (CGRP) and vasoactive intestinal 
      polypeptide (VIP) and compared these responses to human mast cell responses to 
      immunoglobulin E (IgE)/anti-IgE and compound 48/80. Primary cultured mast cells, 
      generated from CD34(+) progenitors in the presence of stem cell factor and 
      interleukin-6 (IL-6), and human cultured mast cells (LAD2) were stimulated with 
      these and other stimuli (gastrin, concanavalin A, radiocontrast media, and 
      mannitol) and their degranulation and chemokine production was assessed. VIP and 
      SP stimulated primary human mast cells and LAD cells to degranulate; gastrin, 
      concanavalin A, radiocontrast media, mannitol, CGRP and NGF did not activate 
      degranulation. While anti-IgE stimulation did not induce significant production 
      of chemokines, stimulation with VIP, SP or compound 48/80 potently induced 
      production of monocyte chemoattractant protein-1, inducible protein-10, monokine 
      induced by interferon-gamma (MIG), RANTES (regulated on activation, normal, 
      T-cell expressed, and secreted) and IL-8. VIP, SP and compound 48/80 also 
      activated release of tumour necrosis factor, IL-3 and granulocyte-macrophage 
      colony-stimulating factor, but not IL-4, interferon-gamma or eotaxin. Human mast 
      cells expressed surface neurokinin 1 receptor (NK1R), NK2R, NK3R and VIP receptor 
      type 2 (VPAC2) but not VPAC1 and activation of human mast cells by IgE/anti-IgE 
      up-regulated expression of VPAC2, NK2R, and NK3R. These studies demonstrate the 
      pattern of receptor expression and activation of mast cell by a host of G-protein 
      coupled receptor ligands and suggest that SP and VIP activate a unique signalling 
      pathway in human mast cells. These results are likely to have direct relevance to 
      neuronally induced inflammatory diseases.
FAU - Kulka, Marianna
AU  - Kulka M
AD  - National Research Council Canada, Room 432, 550 University Avenue, Charlottetown, 
      PE, Canada. marianna.kulka@nrc-cnrc.gc.ca
FAU - Sheen, Cecilia H
AU  - Sheen CH
FAU - Tancowny, Brian P
AU  - Tancowny BP
FAU - Grammer, Leslie C
AU  - Grammer LC
FAU - Schleimer, Robert P
AU  - Schleimer RP
LA  - eng
GR  - R01 HL068546/HL/NHLBI NIH HHS/United States
GR  - R01 HL078860/HL/NHLBI NIH HHS/United States
GR  - R01HL078860/HL/NHLBI NIH HHS/United States
GR  - R01HL068546/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20071006
PL  - England
TA  - Immunology
JT  - Immunology
JID - 0374672
RN  - 0 (Chemokines)
RN  - 0 (Elafin)
RN  - 0 (Neuropeptides)
RN  - 0 (PI3 protein, human)
RN  - 0 (Receptors, Neurokinin-1)
RN  - 0 (Receptors, Neuropeptide)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 33507-63-0 (Substance P)
RN  - 37221-79-7 (Vasoactive Intestinal Peptide)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 9061-61-4 (Nerve Growth Factor)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
RN  - JHB2QIZ69Z (Calcitonin Gene-Related Peptide)
SB  - IM
MH  - Calcitonin Gene-Related Peptide/immunology
MH  - Cell Degranulation/immunology
MH  - Cells, Cultured
MH  - Chemokines/*biosynthesis
MH  - Elafin/antagonists & inhibitors
MH  - GTP-Binding Proteins/antagonists & inhibitors
MH  - Humans
MH  - Immunoglobulin E/immunology
MH  - Mast Cells/*immunology
MH  - Nerve Growth Factor/immunology
MH  - Neuropeptides/*immunology
MH  - Receptors, Neurokinin-1/metabolism
MH  - Receptors, Neuropeptide/metabolism
MH  - Signal Transduction/immunology
MH  - Substance P/immunology
MH  - Tumor Necrosis Factor-alpha/biosynthesis
MH  - Vasoactive Intestinal Peptide/immunology
PMC - PMC2433325
EDAT- 2007/10/10 09:00
MHDA- 2008/03/28 09:00
PMCR- 2009/03/01
CRDT- 2007/10/10 09:00
PHST- 2007/10/10 09:00 [pubmed]
PHST- 2008/03/28 09:00 [medline]
PHST- 2007/10/10 09:00 [entrez]
PHST- 2009/03/01 00:00 [pmc-release]
AID - IMM2705 [pii]
AID - 10.1111/j.1365-2567.2007.02705.x [doi]
PST - ppublish
SO  - Immunology. 2008 Mar;123(3):398-410. doi: 10.1111/j.1365-2567.2007.02705.x. Epub 
      2007 Oct 6.