PMID- 17923043
OWN - NLM
STAT- MEDLINE
DCOM- 20081203
LR  - 20181201
IS  - 0376-2491 (Print)
IS  - 0376-2491 (Linking)
VI  - 87
IP  - 28
DP  - 2007 Jul 24
TI  - [Protein kinase C-betaI, betaII in mouse diabetic nephropathy kidney and its 
      relation to nephroprotective actions of the angiotensin receptor blocker 
      telmisartan].
PG  - 1991-5
AB  - OBJECTIVE: To investigate the localization and expression of protein kinase C 
      (PKC)-betaI, betaII in diabetic nephropathy (DN) mouse kidney and its relation to 
      angiotensin receptor blocker telmisartan (Micardis). METHODS: Eighteen mice were 
      divided into three groups: normal group, DN group and Micardis-treated group (n = 
      6, each group). The expression of PKC-betaI, betaII, transforming growth factor- 
      beta 1 (TGF-beta1) and vascular endothelial growth factor (VEGF) in glomeruli was 
      measured by semiquantitative immunofluorescence histochemistry, the localization 
      of PKC-betaI, betaII was detected by confocal immunofluorescence laser scanning 
      microscopy and the expression of PKC-betaI, betaII in renal cortex, outer and 
      inner medulla were evaluated by semiquantitative Western blotting. RESULTS: 
      Compared to normal mice, the expression of PKC-betaI and betaII on apical 
      membrane of proximal tubule epithelial cells of DN mice was significantly 
      increased, whereas the expression of PKC-betaII on cortical and inner medullary 
      collecting duct was decreased. Western blotting detected increasing expression of 
      PKC-betaI in the renal cortex and outer medulla (P < 0.01), and decreasing 
      expression of PKC-betaII in renal cortex of DN mice (P < 0.01). Enhanced 
      expression of PKC-betaI as well as TGF-beta1 and VEGF (P < 0.01) were shown in 
      the glomeruli of DN mice, where the expression of PKC-betaII was decreased (P < 
      0.05). Meanwhile, PKC-betaI exhibited a positive correlation to TGF-beta1 (r = 
      0.649, P = 0.030), but no correlation to VEGF (r = 0.387, P = 0.079). Micardis 
      could partly attenuate above changes. CONCLUSION: The localization and expression 
      of PKC-betaI, betaII are altered in DN mice, PKC-betaI, betaII may change the 
      function of proximal tubule and PKC-betaI may contribute to glomerular 
      hypertrophy through influencing the expression of glomerular TGF-beta1. Treatment 
      with Micardis can partly improve the abnormal expression and distribution of 
      PKC-betaI, betaII in kidneys of DN mice, which suggests that 
      renin-angiotensin-system is implicated in the pathogenesis of DN by regulating 
      the expression and activation of PKC-betaI, betaII isoforms.
FAU - Yao, Li-jun
AU  - Yao LJ
AD  - Department of Nephrology, Union Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan 430030, China.
FAU - Wang, Jian-qing
AU  - Wang JQ
FAU - Deng, An-guo
AU  - Deng AG
FAU - Liu, Jian-she
AU  - Liu JS
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhonghua Yi Xue Za Zhi
JT  - Zhonghua yi xue za zhi
JID - 7511141
RN  - 0 (Angiotensin II Type 1 Receptor Blockers)
RN  - 0 (Benzimidazoles)
RN  - 0 (Benzoates)
RN  - 0 (Isoenzymes)
RN  - 0 (Transforming Growth Factor beta1)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - EC 2.7.11.13 (Protein Kinase C)
RN  - U5SYW473RQ (Telmisartan)
SB  - IM
MH  - Angiotensin II Type 1 Receptor Blockers/*pharmacology
MH  - Animals
MH  - Benzimidazoles/*pharmacology
MH  - Benzoates/*pharmacology
MH  - Diabetic Nephropathies/enzymology/pathology/*prevention & control
MH  - Fluorescent Antibody Technique
MH  - Isoenzymes/metabolism
MH  - Kidney/*drug effects/enzymology/pathology
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Protein Kinase C/*metabolism
MH  - Telmisartan
MH  - Transforming Growth Factor beta1/metabolism
MH  - Vascular Endothelial Growth Factor A/metabolism
EDAT- 2007/10/10 09:00
MHDA- 2008/12/17 09:00
CRDT- 2007/10/10 09:00
PHST- 2007/10/10 09:00 [pubmed]
PHST- 2008/12/17 09:00 [medline]
PHST- 2007/10/10 09:00 [entrez]
PST - ppublish
SO  - Zhonghua Yi Xue Za Zhi. 2007 Jul 24;87(28):1991-5.