PMID- 17923118
OWN - NLM
STAT- MEDLINE
DCOM- 20071206
LR  - 20191210
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 363
IP  - 4
DP  - 2007 Nov 30
TI  - Stimulation by glutamine and proline of HGF production in hepatic stellate cells.
PG  - 978-82
AB  - Amino acids regulate cellular functions in a variety of cell types. Most notably, 
      leucine stimulates protein production through the mammalian target of rapamycin 
      (mTOR)-dependent signaling pathway. We investigated the effect of amino acids on 
      hepatocyte growth factor (HGF) production. Treatment with glutamine and proline, 
      as well as leucine, increased HGF levels in the culture medium of a rat hepatic 
      stellate cell clone in a dose-dependent manner. Up-regulation of phosphorylation 
      of 70 kDa ribosomal protein S6 kinase and eukaryotic initiation factor 4E-binding 
      protein 1 was not apparent in the cells after treatment with glutamine or 
      proline. When rats received injections of glutamine or proline, hepatic and 
      circulating HGF levels increased and peaked around 12h after treatment. Glutamine 
      and proline may have the potential to stimulate HGF production but the mechanism 
      underlying this stimulation seems not to be through the mTOR-dependent signaling 
      pathway.
FAU - Nishikawa, Takako
AU  - Nishikawa T
AD  - Department of Gastroenterology, Faculty of Medicine, University of Tokyo, Hongo 
      7-3-1, Bunkyo-ku, Tokyo 113-8655, Japan.
FAU - Tomiya, Tomoaki
AU  - Tomiya T
FAU - Ohtomo, Natsuko
AU  - Ohtomo N
FAU - Inoue, Yukiko
AU  - Inoue Y
FAU - Ikeda, Hitoshi
AU  - Ikeda H
FAU - Tejima, Kazuaki
AU  - Tejima K
FAU - Watanabe, Naoko
AU  - Watanabe N
FAU - Tanoue, Yasushi
AU  - Tanoue Y
FAU - Omata, Masao
AU  - Omata M
FAU - Fujiwara, Kenji
AU  - Fujiwara K
LA  - eng
PT  - Journal Article
DEP - 20071001
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Carrier Proteins)
RN  - 0 (Culture Media)
RN  - 0 (Eif4ebp1 protein, rat)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Phosphoproteins)
RN  - 0RH81L854J (Glutamine)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - 9DLQ4CIU6V (Proline)
RN  - EC 2.7.11.1 (Ribosomal Protein S6 Kinases, 70-kDa)
SB  - IM
MH  - Animals
MH  - Carrier Proteins/metabolism
MH  - Cell Line
MH  - Cell Separation
MH  - Culture Media
MH  - Glutamine/*pharmacology
MH  - Hepatocyte Growth Factor/*biosynthesis
MH  - Hepatocytes/*drug effects/*metabolism
MH  - Intracellular Signaling Peptides and Proteins
MH  - Male
MH  - Phosphoproteins/metabolism
MH  - Phosphorylation/drug effects
MH  - Proline/*pharmacology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Ribosomal Protein S6 Kinases, 70-kDa/metabolism
EDAT- 2007/10/10 09:00
MHDA- 2007/12/07 09:00
CRDT- 2007/10/10 09:00
PHST- 2007/09/06 00:00 [received]
PHST- 2007/09/20 00:00 [accepted]
PHST- 2007/10/10 09:00 [pubmed]
PHST- 2007/12/07 09:00 [medline]
PHST- 2007/10/10 09:00 [entrez]
AID - S0006-291X(07)02075-X [pii]
AID - 10.1016/j.bbrc.2007.09.082 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2007 Nov 30;363(4):978-82. doi: 
      10.1016/j.bbrc.2007.09.082. Epub 2007 Oct 1.