PMID- 17925605
OWN - NLM
STAT- MEDLINE
DCOM- 20080114
LR  - 20220310
IS  - 0954-6928 (Print)
IS  - 0954-6928 (Linking)
VI  - 18
IP  - 7
DP  - 2007 Nov
TI  - Monocyte-derived dendritic cells of patients with coronary artery disease show an 
      increased expression of costimulatory molecules CD40, CD80 and CD86 in vitro.
PG  - 523-31
AB  - BACKGROUND: Atherosclerosis is a disease triggered by diverse exogenous stimuli 
      and sustained by chronic inflammatory processes. Dendritic cells (DCs) are key 
      regulatory antigen-presenting cells and play a crucial role in regulating the 
      adaptive and innate immune system in any chronic inflammatory process. DCs are 
      present in atherosclerotic lesions in the areas of the highest T-cell density. So 
      far, their role in atherosclerosis has not been fully elucidated. We investigated 
      the phenotypic properties of DCs in patients with coronary artery disease (CAD) 
      in comparison to healthy individuals. METHODS: Peripheral blood monocytes were 
      isolated from 50 patients with CAD and 19 healthy individuals and differentiated 
      over 9 days to immature and mature DCs. Analysis of the distribution of important 
      stimulatory and costimulatory molecules on the surface of immature and mature DCs 
      was performed by flow cytometry. RESULTS: We observed no changes between the 
      groups concerning cell numbers or expression of CD1a or HLA-DR on DCs. Patients 
      with CAD, however, showed a significant upregulation of the costimulatory 
      molecules CD80, CD86 and CD40 as compared with healthy controls. Expression of 
      CD40, CD80 and CD86 on DCs partly correlated with smoking, family history of CAD, 
      as well as with C-reactive protein levels. High-density lipoprotein cholesterol 
      was inversely associated with the expression of CD40 and CD80 on mature DCs 
      (P<0.05). CONCLUSION: Upregulation of important costimulatory molecules on 
      monocyte-derived DCs of CAD patients, is influenced multifactorially. Our results 
      show notable differences between CAD patients and healthy individuals, possibly 
      contributing to the pathophysiological processes in atherogenesis.
FAU - Dopheide, Jorn F
AU  - Dopheide JF
AD  - Department of Medicine II, Johannes-Gutenberg University, Mainz, Germany. 
      dopheide@2-med.klinik.uni-mainz.de
FAU - Sester, Urban
AU  - Sester U
FAU - Schlitt, Axel
AU  - Schlitt A
FAU - Horstick, Georg
AU  - Horstick G
FAU - Rupprecht, Hans J
AU  - Rupprecht HJ
FAU - Munzel, Thomas
AU  - Munzel T
FAU - Blankenberg, Stefan
AU  - Blankenberg S
LA  - eng
PT  - Journal Article
PL  - England
TA  - Coron Artery Dis
JT  - Coronary artery disease
JID - 9011445
RN  - 0 (B7-1 Antigen)
RN  - 0 (B7-2 Antigen)
RN  - 0 (CD40 Antigens)
RN  - 9007-41-4 (C-Reactive Protein)
SB  - IM
MH  - Aged
MH  - Atherosclerosis/pathology
MH  - B7-1 Antigen/*biosynthesis
MH  - B7-2 Antigen/*biosynthesis
MH  - C-Reactive Protein/metabolism
MH  - CD40 Antigens/*biosynthesis
MH  - Cell Differentiation
MH  - Coronary Artery Disease/*blood/pathology
MH  - Dendritic Cells/cytology/*metabolism
MH  - Flow Cytometry/methods
MH  - *Gene Expression Regulation
MH  - Humans
MH  - Leukocytes, Mononuclear/metabolism
MH  - Middle Aged
MH  - Monocytes/*metabolism
MH  - Risk Factors
EDAT- 2007/10/11 09:00
MHDA- 2008/01/15 09:00
CRDT- 2007/10/11 09:00
PHST- 2007/10/11 09:00 [pubmed]
PHST- 2008/01/15 09:00 [medline]
PHST- 2007/10/11 09:00 [entrez]
AID - 00019501-200711000-00004 [pii]
AID - 10.1097/MCA.0b013e3282eff1ad [doi]
PST - ppublish
SO  - Coron Artery Dis. 2007 Nov;18(7):523-31. doi: 10.1097/MCA.0b013e3282eff1ad.