PMID- 17927584
OWN - NLM
STAT- MEDLINE
DCOM- 20071226
LR  - 20191210
IS  - 0309-0167 (Print)
IS  - 0309-0167 (Linking)
VI  - 51
IP  - 5
DP  - 2007 Nov
TI  - Epidermal growth factor receptor (EGFR) gene copy number detection in 
      non-small-cell lung cancer; a comparison of fluorescence in situ hybridization 
      and chromogenic in situ hybridization.
PG  - 631-7
AB  - AIMS: The epidermal growth factor receptor (EGFR) is an important target for 
      anticancer therapy. In non-small-cell lung cancer (NSCLC), mutations in the 
      tyrosine kinase domain of EGFR and EGFR gene copy number have been demonstrated 
      to identify patients most likely to benefit from EGFR tyrosine kinase inhibitors. 
      EGFR gene copy number has been assessed mainly by fluorescence in situ 
      hybridization (FISH), a method requiring the use of a fluorescence microscope and 
      often hampered by the rapid fading of the fluorescent signal. These limitations 
      of FISH can be overcome by using chromogenic in situ hybridization (CISH). To 
      test the applicability of CISH for EGFR gene copy number testing in NSCLC, a 
      comparison of CISH and FISH was performed. METHODS AND RESULTS: A total of 58 
      formalin-fixed, frozen NSCLC tissue samples were collected on which both CISH and 
      FISH were performed. High concordance was found in the assessment of EGFR copy 
      number between observers and between techniques (kappa coefficient = 0.64-0.76). 
      CISH seemed the ideal technique for paraffin sections, whereas FISH was 
      favourable for frozen material. CONCLUSIONS: CISH is a suitable alternative 
      strategy to FISH in determining EGFR gene copy number in NSCLC patients.
FAU - Gallegos Ruiz, M I
AU  - Gallegos Ruiz MI
AD  - Department of Medical Oncology, VU University Medical Centre, Amsterdam, The 
      Netherlands.
FAU - Floor, K
AU  - Floor K
FAU - Vos, W
AU  - Vos W
FAU - Grunberg, K
AU  - Grunberg K
FAU - Meijer, G A
AU  - Meijer GA
FAU - Rodriguez, J A
AU  - Rodriguez JA
FAU - Giaccone, G
AU  - Giaccone G
LA  - eng
PT  - Comparative Study
PT  - Evaluation Study
PT  - Journal Article
PL  - England
TA  - Histopathology
JT  - Histopathology
JID - 7704136
RN  - 0 (Chromogenic Compounds)
RN  - EC 2.7.10.1 (ErbB Receptors)
SB  - IM
MH  - Carcinoma, Non-Small-Cell Lung/*diagnosis
MH  - Chromogenic Compounds
MH  - ErbB Receptors/*genetics
MH  - *Gene Dosage
MH  - Humans
MH  - In Situ Hybridization/*methods
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Lung Neoplasms/*diagnosis
EDAT- 2007/10/12 09:00
MHDA- 2007/12/27 09:00
CRDT- 2007/10/12 09:00
PHST- 2007/10/12 09:00 [pubmed]
PHST- 2007/12/27 09:00 [medline]
PHST- 2007/10/12 09:00 [entrez]
AID - HIS2854 [pii]
AID - 10.1111/j.1365-2559.2007.02854.x [doi]
PST - ppublish
SO  - Histopathology. 2007 Nov;51(5):631-7. doi: 10.1111/j.1365-2559.2007.02854.x.