PMID- 17928083
OWN - NLM
STAT- MEDLINE
DCOM- 20071221
LR  - 20071026
IS  - 0168-1656 (Print)
IS  - 0168-1656 (Linking)
VI  - 132
IP  - 2
DP  - 2007 Oct 31
TI  - In vitro evaluation of the behaviour of human polymorphonuclear neutrophils in 
      direct contact with chitosan-based membranes.
PG  - 218-26
AB  - Several novel biodegradable materials have been proposed for wound healing 
      applications in the past few years. Taking into consideration the 
      biocompatibility of chitosan-based biomaterials, and that they promote adequate 
      cell adhesion, this work aims at investigating the effect of chitosan-based 
      membranes, over the activation of human polymorphonuclear neutrophils (PMNs). The 
      recruitment and activation of polymorphonuclear neutrophils (PMNs) reflects a 
      primary reaction to foreign bodies. Activation of neutrophils results in the 
      production of reactive oxygen species (ROS) such as O(2)(-) and HO(-) and the 
      release of hydrolytic enzymes which are determinant factors in the inflammatory 
      process, playing an essential role in the healing mechanisms. PMNs isolated from 
      human peripheral blood of healthy volunteers were cultured in the presence of 
      chitosan or chitosan/soy newly developed membranes. The effect of the 
      biomaterials on the activation of PMNs was assessed by the quantification of 
      lysozyme and ROS. The results showed that PMNs, in the presence of the 
      chitosan-based membranes secrete similar lysozyme amounts, as compared to 
      controls (PMNs without materials) and also showed that the materials do not 
      stimulate the production of either O(2)(-) or HO(-). Moreover, PMNs incubated 
      with the biomaterials when stimulated with phorbol 12-myristate 13-acetate (PMA) 
      or formyl-methionyl-leucyl-phenylalanine (fMLP) showed a chemiluminescence 
      profile with a slightly lower intensity, to that observed for positive controls 
      (cells without materials and stimulated with PMA), which reflects the maintenance 
      of their stimulation capacity. Our data suggests that the new biomaterials 
      studied herein do not elicit activation of PMNs, as assessed by the low lysozyme 
      activity and by the minor detection of ROS by chemiluminescence. These findings 
      reinforce previous statements supporting the suitability of chitosan-based 
      materials for wound healing applications.
FAU - Santos, T C
AU  - Santos TC
AD  - 3B's Research Group - Biomaterials, Biodegradables and Biomimetics, Department of 
      Polymer Engineering, Campus de Gualtar, 4710-057 Braga, Portugal.
FAU - Marques, A P
AU  - Marques AP
FAU - Silva, S S
AU  - Silva SS
FAU - Oliveira, J M
AU  - Oliveira JM
FAU - Mano, J F
AU  - Mano JF
FAU - Castro, A G
AU  - Castro AG
FAU - Reis, R L
AU  - Reis RL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070712
PL  - Netherlands
TA  - J Biotechnol
JT  - Journal of biotechnology
JID - 8411927
RN  - 0 (Biocompatible Materials)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Soybean Proteins)
RN  - 9012-76-4 (Chitosan)
RN  - EC 3.2.1.17 (Muramidase)
SB  - IM
MH  - Biocompatible Materials/*pharmacology
MH  - Cells, Cultured
MH  - Chitosan/*pharmacology
MH  - Humans
MH  - *Materials Testing
MH  - Muramidase/metabolism
MH  - Neutrophil Activation/*drug effects/physiology
MH  - Neutrophils/*metabolism
MH  - Reactive Oxygen Species/metabolism
MH  - Soybean Proteins/*pharmacology
MH  - Tissue Scaffolds
MH  - Wound Healing
EDAT- 2007/10/12 09:00
MHDA- 2007/12/22 09:00
CRDT- 2007/10/12 09:00
PHST- 2007/01/02 00:00 [received]
PHST- 2007/06/22 00:00 [revised]
PHST- 2007/07/06 00:00 [accepted]
PHST- 2007/10/12 09:00 [pubmed]
PHST- 2007/12/22 09:00 [medline]
PHST- 2007/10/12 09:00 [entrez]
AID - S0168-1656(07)00988-1 [pii]
AID - 10.1016/j.jbiotec.2007.07.497 [doi]
PST - ppublish
SO  - J Biotechnol. 2007 Oct 31;132(2):218-26. doi: 10.1016/j.jbiotec.2007.07.497. Epub 
      2007 Jul 12.