PMID- 1792900
OWN - NLM
STAT- MEDLINE
DCOM- 19920331
LR  - 20190912
IS  - 0374-5600 (Print)
IS  - 0374-5600 (Linking)
VI  - 33
IP  - 4
DP  - 1991 Aug
TI  - Neuronal differentiation of Ewing's sarcoma induced by cholera toxin B and 
      bromodeoxyuridine--establishment of Ewing's sarcoma cell line and histochemical 
      study.
PG  - 428-33
AB  - An Ewing's sarcoma (ES) cell line was established from a metastatic bone marrow 
      specimen in a patient with advanced disease, and some histochemical 
      characteristics were investigated by neuronal differentiation induced with 
      cholera toxin B (CTB) and bromodeoxyuridine (BrdU). Neuronal differentiation was 
      investigated by the expression of neurofilament and Leu-7, and glial 
      differentiation was observed by expression of S-100 protein. Neurofilament (NF) 
      and Leu-7 were positive in ES cells and these were expressed more intensively by 
      induction with CTB than with BrdU. There was no expression of S-100 protein in 
      untreated or differentiated ES cells. ES cells became differentiated to neuronal 
      cells with CTB and BrdU, but it was not observed, that ES cells had the potential 
      to differentiate to glial cells. It appears that ES is of more primitive neural 
      origin than neuroblastoma, primitive neuroectodermal tumors and other related 
      neural tumors.
FAU - Ohta, S
AU  - Ohta S
AD  - Department of Pediatrics, Shiga University of Medical Science, Japan.
FAU - Suzuki, A
AU  - Suzuki A
FAU - Shimada, M
AU  - Shimada M
FAU - Kosuga, M
AU  - Kosuga M
FAU - Taga, T
AU  - Taga T
FAU - Sugiura, Y
AU  - Sugiura Y
FAU - Iwai, M
AU  - Iwai M
FAU - Miyahira, Y
AU  - Miyahira Y
FAU - Okabe, H
AU  - Okabe H
FAU - Suzuki, S
AU  - Suzuki S
AU  - et al.
LA  - eng
PT  - Journal Article
PL  - Australia
TA  - Acta Paediatr Jpn
JT  - Acta paediatrica Japonica : Overseas edition
JID - 0370357
RN  - 0 (Antigens, Differentiation)
RN  - 0 (S100 Proteins)
RN  - 9012-63-9 (Cholera Toxin)
RN  - G34N38R2N1 (Bromodeoxyuridine)
SB  - IM
MH  - Antigens, Differentiation/analysis
MH  - Bromodeoxyuridine
MH  - Cell Differentiation/drug effects
MH  - Child
MH  - Cholera Toxin
MH  - Humans
MH  - Intermediate Filaments/pathology
MH  - Male
MH  - Neurons/chemistry/*cytology
MH  - S100 Proteins/analysis
MH  - Sarcoma, Ewing/chemistry/*pathology/physiopathology
MH  - Tumor Cells, Cultured/chemistry/cytology
EDAT- 1991/08/01 00:00
MHDA- 1991/08/01 00:01
CRDT- 1991/08/01 00:00
PHST- 1991/08/01 00:00 [pubmed]
PHST- 1991/08/01 00:01 [medline]
PHST- 1991/08/01 00:00 [entrez]
AID - 10.1111/j.1442-200x.1991.tb02566.x [doi]
PST - ppublish
SO  - Acta Paediatr Jpn. 1991 Aug;33(4):428-33. doi: 
      10.1111/j.1442-200x.1991.tb02566.x.