PMID- 17931409
OWN - NLM
STAT- MEDLINE
DCOM- 20080404
LR  - 20220311
IS  - 1742-4690 (Electronic)
IS  - 1742-4690 (Linking)
VI  - 4
DP  - 2007 Oct 11
TI  - Rapid spread of mouse mammary tumor virus in cultured human breast cells.
PG  - 73
AB  - BACKGROUND: The role of mouse mammary tumor virus (MMTV) as a causative agent in 
      human breast carcinogenesis has recently been the subject of renewed interest. 
      The proposed model is based on the detection of MMTV sequences in human breast 
      cancer but not in healthy breast tissue. One of the main drawbacks to this model, 
      however, was that until now human cells had not been demonstrated to sustain 
      productive MMTV infection. RESULTS: Here, we show for the first time the rapid 
      spread of mouse mammary tumor virus, MMTV(GR), in cultured human mammary cells 
      (Hs578T), ultimately leading to the infection of every cell in culture. The 
      replication of the virus was monitored by quantitative PCR, quantitative RT-PCR 
      and immunofluorescence imaging. The infected human cells expressed, upon 
      cultivation with dexamethasone, MMTV structural proteins and released spiked 
      B-type virions, the infectivity of which could be neutralized by anti-MMTV 
      antibody. Replication of the virus was efficiently blocked by an inhibitor of 
      reverse transcription, 3'-azido-3'-deoxythymidine. The human origin of the 
      infected cells was confirmed by determining a number of integration sites hosting 
      the provirus, which were unequivocally identified as human sequences. CONCLUSION: 
      Taken together, our results show that human cells can support replication of 
      mouse mammary tumor virus.
FAU - Indik, Stanislav
AU  - Indik S
AD  - Research Institute for Virology and Biomedicine, University of Veterinary 
      Medicine Vienna, Vienna, A-1210, Austria. stanislav.indik@vu-wien.ac.at
FAU - Gunzburg, Walter H
AU  - Gunzburg WH
FAU - Kulich, Pavel
AU  - Kulich P
FAU - Salmons, Brian
AU  - Salmons B
FAU - Rouault, Francoise
AU  - Rouault F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20071011
PL  - England
TA  - Retrovirology
JT  - Retrovirology
JID - 101216893
RN  - 0 (DNA, Viral)
RN  - 4B9XT59T7S (Zidovudine)
SB  - IM
MH  - Animals
MH  - Breast Neoplasms/*virology
MH  - Cell Line
MH  - DNA, Viral/analysis/genetics
MH  - Humans
MH  - Mammary Tumor Virus, Mouse/*drug effects/genetics/physiology
MH  - Polymerase Chain Reaction/methods
MH  - Tumor Virus Infections/immunology
MH  - Virus Replication/drug effects
MH  - Zidovudine/pharmacology
PMC - PMC2169256
EDAT- 2007/10/13 09:00
MHDA- 2008/04/05 09:00
PMCR- 2007/10/11
CRDT- 2007/10/13 09:00
PHST- 2007/05/31 00:00 [received]
PHST- 2007/10/11 00:00 [accepted]
PHST- 2007/10/13 09:00 [pubmed]
PHST- 2008/04/05 09:00 [medline]
PHST- 2007/10/13 09:00 [entrez]
PHST- 2007/10/11 00:00 [pmc-release]
AID - 1742-4690-4-73 [pii]
AID - 10.1186/1742-4690-4-73 [doi]
PST - epublish
SO  - Retrovirology. 2007 Oct 11;4:73. doi: 10.1186/1742-4690-4-73.