PMID- 17932758
OWN - NLM
STAT- MEDLINE
DCOM- 20080805
LR  - 20211020
IS  - 0163-2116 (Print)
IS  - 0163-2116 (Linking)
VI  - 53
IP  - 6
DP  - 2008 Jun
TI  - The effect of PPARalpha and PPARgamma ligands on inflammation and ABCA1 
      expression in cultured gallbladder epithelial cells.
PG  - 1707-15
AB  - The preservation of gallbladder function by control of inflammation and 
      elimination of cholesterol accumulation in gallbladder epithelial cells (GBEC) 
      could contribute to the prevention of gallstone formation and cholecystitis. 
      Peroxisome proliferator-activated receptors (PPARs) modulate inflammation and 
      lipid metabolism in various cells and GBEC efflux of excessive amounts of 
      absorbed cholesterol through the ATP-binding cassette transporter A1 
      (ABCA1)-mediated pathway. The aim of this study was to determine whether ligands 
      of PPARalpha and PPARgamma modulate inflammation and have an effect on ABCA1 
      expression in GBEC. Canine GBEC were cultured on dishes coated with collagen 
      matrix. We performed Western blot analysis for the expression of specific protein 
      and/or RT-PCR for the expression of specific mRNA. PPARalpha and PPARgamma 
      expression was observed and increased in GBEC treated with WY-14643 (PPARalpha 
      ligand), troglitazone (PPARgamma ligand), and lipopolysaccharide (LPS) compared 
      to the no-treatment control and PPARalpha( antagonist (GW-9662) treatment group. 
      WY-14643, troglitazone, and LPS also induced an increase in the expression of 
      ABCA1 protein and mRNA in cultured GBEC. LPS-induced TNFalpha mRNA expression was 
      suppressed by pretreatment with WY-14643 and troglitazone preceding LPS treatment 
      in GBEC. PPAR ligands, especially PPARgamma, may preserve gallbladder function by 
      suppression of inflammatory reaction and prevention of cholesterol accumulation 
      in GBEC, contributing to the prevention of gallstone formation and progression to 
      cholecystitis.
FAU - Lee, Jin
AU  - Lee J
AD  - Division of Gastroenterology, Department of Internal Medicine, Hallym University 
      Hangang Sacred Heart Hospital, 94-200, Youngdungpo-Dong, Youngdungpo-Gu, Seoul 
      150-030, South Korea. jinlee@medimail.co.kr
FAU - Hong, Eun Mi
AU  - Hong EM
FAU - Byun, Hyun Woo
AU  - Byun HW
FAU - Choi, Min Ho
AU  - Choi MH
FAU - Jang, Hyun Joo
AU  - Jang HJ
FAU - Eun, Chang Soo
AU  - Eun CS
FAU - Kae, Sea Hyub
AU  - Kae SH
FAU - Choi, Ho Soon
AU  - Choi HS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Dig Dis Sci
JT  - Digestive diseases and sciences
JID - 7902782
RN  - 0 (ATP Binding Cassette Transporter 1)
RN  - 0 (ATP-Binding Cassette Transporters)
RN  - 0 (Ligands)
RN  - 0 (PPAR alpha)
RN  - 0 (PPAR gamma)
SB  - IM
MH  - ATP Binding Cassette Transporter 1
MH  - ATP-Binding Cassette Transporters/*metabolism
MH  - Analysis of Variance
MH  - Animals
MH  - Blotting, Western
MH  - Cells, Cultured
MH  - Dogs
MH  - Epithelial Cells/cytology/metabolism
MH  - Gallbladder/*cytology/*metabolism
MH  - Inflammation/*metabolism
MH  - Ligands
MH  - PPAR alpha/*pharmacology
MH  - PPAR gamma/*pharmacology
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 2007/10/13 09:00
MHDA- 2008/08/06 09:00
CRDT- 2007/10/13 09:00
PHST- 2007/03/08 00:00 [received]
PHST- 2007/09/19 00:00 [accepted]
PHST- 2007/10/13 09:00 [pubmed]
PHST- 2008/08/06 09:00 [medline]
PHST- 2007/10/13 09:00 [entrez]
AID - 10.1007/s10620-007-0029-5 [doi]
PST - ppublish
SO  - Dig Dis Sci. 2008 Jun;53(6):1707-15. doi: 10.1007/s10620-007-0029-5.