PMID- 1793452
OWN - NLM
STAT- MEDLINE
DCOM- 19920330
LR  - 20190718
IS  - 0021-9150 (Print)
IS  - 0021-9150 (Linking)
VI  - 89
IP  - 2-3
DP  - 1991 Aug
TI  - Tumor necrosis factor-alpha in human arterial wall with atherosclerosis.
PG  - 247-54
AB  - Tumor necrosis factor-alpha (TNF-alpha) was demonstrated in human normal and 
      atherosclerotic aorta, iliac and femoral arteries by immunohistochemistry using 
      monoclonal and polyclonal antibodies. TNF was present in the cells of the 
      arterial wall and as granular and diffuse extracellular deposits in the 
      connective tissue matrix. Quantitative determinations of TNF by ELISA showed mean 
      values of 21.7 +/- 0.7 ng/100 mg total extracted protein in normal intima, 38.2 
      +/- 0.5 in intimal thickenings, 25.5 +/- 1.1 in fibrous plaques and 16.8 +/- 0.2 
      ng/100 mg total extracted protein in media. Intimal thickenings presented the 
      highest amounts of TNF with a statistically significant difference when compared 
      to normal intima (P less than 0.05) and media (P less than 0.01). TNF-alpha 
      concentrations in arterial eluates were about 200 times higher than in the 
      corresponding serum samples. Western blotting analysis confirmed TNF-alpha eluted 
      from the arterial wall to be about 17 kDa similar to human recombinant TNF-alpha. 
      TNF-alpha in human atherosclerotic wall could be actively involved in the 
      inflammatory events associated with atherosclerosis.
FAU - Rus, H G
AU  - Rus HG
AD  - Medical Clinic No. 1, Cluj-Napoca, Romania.
FAU - Niculescu, F
AU  - Niculescu F
FAU - Vlaicu, R
AU  - Vlaicu R
LA  - eng
PT  - Journal Article
PL  - Ireland
TA  - Atherosclerosis
JT  - Atherosclerosis
JID - 0242543
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Aorta/metabolism
MH  - Arteries/*metabolism
MH  - Arteriosclerosis/*metabolism
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Femoral Artery/metabolism
MH  - Humans
MH  - Iliac Artery/metabolism
MH  - Immunoblotting
MH  - Immunohistochemistry
MH  - Tumor Necrosis Factor-alpha/*metabolism
EDAT- 1991/08/01 00:00
MHDA- 1991/08/01 00:01
CRDT- 1991/08/01 00:00
PHST- 1991/08/01 00:00 [pubmed]
PHST- 1991/08/01 00:01 [medline]
PHST- 1991/08/01 00:00 [entrez]
AID - 0021-9150(91)90066-C [pii]
AID - 10.1016/0021-9150(91)90066-c [doi]
PST - ppublish
SO  - Atherosclerosis. 1991 Aug;89(2-3):247-54. doi: 10.1016/0021-9150(91)90066-c.