PMID- 17934749
OWN - NLM
STAT- MEDLINE
DCOM- 20080701
LR  - 20211020
IS  - 0721-832X (Print)
IS  - 0721-832X (Linking)
VI  - 246
IP  - 4
DP  - 2008 Apr
TI  - In vivo monosomy 3 detection of posterior uveal melanoma: 3-year follow-up.
PG  - 609-14
AB  - BACKGROUND: Monosomy 3 is a highly specific marker for poor prognosis in 
      posterior uveal melanoma. Unfortunately, cytogenetic prognostication is limited 
      to enucleated eyes or resected tumors. The aim of this study was to evaluate 
      mid-term natural history and safety of in vivo detection of chromosome 3 status 
      in posterior uveal melanomas undergoing plaque brachytherapy. METHODS: A 25-gauge 
      transscleral fine needle aspiration biopsy (FNAB) was performed in 32 eyes 
      affected by posterior uveal melanoma undergoing plaque brachytherapy, just before 
      applying the radioactive plaque. Sampled material underwent fluorescence in situ 
      hybridization (FISH) with centromeric probes for chromosome 3. All patients had a 
      follow-up of at least 36 months. RESULTS: Mean follow-up was 47.1 +/- 8.5 months. 
      Mean largest basal diameter and mean thickness of the tumors were 12.5 +/- 2.7 mm 
      and 8 +/- 2.3 mm respectively. FNAB yielded sufficient material in 26 of 32 cases 
      (81.2%). Adequacy of the sample ranged from 91.1% (ciliary body tumors) to 76.8% 
      (choroidal tumors). Seventeen cases had monosomy 3 (65.3%). No correlation was 
      found between monosomy 3 and tumor dimensions or location (ciliary body vs 
      choroidal tumors). No early and mid-term local complications were documented. 
      Seven patients (21.8%) died during follow-up: five (15.6%) of them died due to 
      metastatic disease (all had monosomy 3 tumors). CONCLUSIONS: Posterior uveal 
      melanomas may be adequately and safely sampled, by intra-operative transscleral 
      FNAB, to detect in vivo monosomy 3.
FAU - Midena, Edoardo
AU  - Midena E
AD  - Department of Ophthalmology, University of Padova, Via Giustiniani 2, 35128 
      Padova, Italy. edoardo.midena@unipd.it
FAU - Bonaldi, Laura
AU  - Bonaldi L
FAU - Parrozzani, Raffaele
AU  - Parrozzani R
FAU - Radin, Pietro P
AU  - Radin PP
FAU - Boccassini, Barbara
AU  - Boccassini B
FAU - Vujosevic, Stela
AU  - Vujosevic S
LA  - eng
PT  - Journal Article
DEP - 20071013
PL  - Germany
TA  - Graefes Arch Clin Exp Ophthalmol
JT  - Graefe's archive for clinical and experimental ophthalmology = Albrecht von 
      Graefes Archiv fur klinische und experimentelle Ophthalmologie
JID - 8205248
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Biopsy, Needle
MH  - Brachytherapy
MH  - Chromosomes, Human, Pair 3/*genetics
MH  - Female
MH  - Follow-Up Studies
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Melanoma/*genetics/pathology/radiotherapy
MH  - Middle Aged
MH  - Monosomy/*genetics
MH  - Prognosis
MH  - Uveal Neoplasms/*genetics/pathology/radiotherapy
EDAT- 2007/10/16 09:00
MHDA- 2008/07/02 09:00
CRDT- 2007/10/16 09:00
PHST- 2007/07/18 00:00 [received]
PHST- 2007/09/12 00:00 [accepted]
PHST- 2007/09/11 00:00 [revised]
PHST- 2007/10/16 09:00 [pubmed]
PHST- 2008/07/02 09:00 [medline]
PHST- 2007/10/16 09:00 [entrez]
AID - 10.1007/s00417-007-0692-4 [doi]
PST - ppublish
SO  - Graefes Arch Clin Exp Ophthalmol. 2008 Apr;246(4):609-14. doi: 
      10.1007/s00417-007-0692-4. Epub 2007 Oct 13.