PMID- 17935071
OWN - NLM
STAT- MEDLINE
DCOM- 20071226
LR  - 20220316
IS  - 0014-2980 (Print)
IS  - 0014-2980 (Linking)
VI  - 37
IP  - 11
DP  - 2007 Nov
TI  - Correlation between recent thymic emigrants and CD31+ (PECAM-1) CD4+ T cells in 
      normal individuals during aging and in lymphopenic children.
PG  - 3270-80
AB  - CD31(+)CD45RA(+)RO(-) lymphocytes contain high numbers of T cell receptor circle 
      (TREC)-bearing T cells; however, the correlation between CD31(+)CD4(+) 
      lymphocytes and TREC during aging and under lymphopenic conditions has not yet 
      been sufficiently investigated. We analyzed TREC, telomere length and telomerase 
      activity within sorted CD31(+) and CD31(-) CD4(+) lymphocytes in healthy 
      individuals from birth to old age. Sorted CD31(+)CD45RA(+)RO(-) naive CD4(+) 
      lymphocytes contained high TREC numbers, whereas CD31(+)CD45RA(-)RO(+) cells 
      (comprising < or =5% of CD4(+) cells during aging) did not contain TREC. CD31(+) 
      overall CD4(+) cells remained TREC rich despite an age-related tenfold reduction 
      from neonatal (100 : 1000) to old age (10 : 1000). Besides a high TREC content, 
      CD31(+)CD45RA(+)RO(-)CD4(+) cells exhibited significantly longer telomeres and 
      higher telomerase activity than CD31(-)CD45RA(+)RO(-)CD4(+) cells, suggesting 
      that CD31(+)CD45RA(+)RO(-)CD4(+) cells represent a distinct population of naive T 
      cells with particularly low replicative history. To analyze the value of CD31 in 
      lymphopenic conditions, we investigated six children after allogeneic 
      hematopoietic stem cell transplantation (HSCT). Reemerging overall CD4(+) as well 
      as naive CD45RA(+)RO(-)CD4(+) cells predominantly expressed CD31 and correlated 
      well with the recurrence of TREC 5-12 months after HSCT. Irrespective of 
      limitations in the elderly, CD31 is an appropriate marker to monitor TREC-rich 
      lymphocytes essentially in lymphopenic children after HSCT.
FAU - Junge, Sonja
AU  - Junge S
AD  - Division of Immunology/Hematology/BMT, University Children's Hospital, Zurich, 
      Switzerland.
FAU - Kloeckener-Gruissem, Barbara
AU  - Kloeckener-Gruissem B
FAU - Zufferey, Romain
AU  - Zufferey R
FAU - Keisker, Andre
AU  - Keisker A
FAU - Salgo, Bettina
AU  - Salgo B
FAU - Fauchere, Jean-Claude
AU  - Fauchere JC
FAU - Scherer, Franziska
AU  - Scherer F
FAU - Shalaby, Tarek
AU  - Shalaby T
FAU - Grotzer, Michael
AU  - Grotzer M
FAU - Siler, Ulrich
AU  - Siler U
FAU - Seger, Reinhard
AU  - Seger R
FAU - Gungor, Tayfun
AU  - Gungor T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Eur J Immunol
JT  - European journal of immunology
JID - 1273201
RN  - 0 (Platelet Endothelial Cell Adhesion Molecule-1)
RN  - EC 2.7.7.49 (Telomerase)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aging/*immunology
MH  - CD4-Positive T-Lymphocytes/cytology/*immunology/metabolism
MH  - Child
MH  - Child, Preschool
MH  - Flow Cytometry
MH  - Humans
MH  - Infant
MH  - Infant, Newborn
MH  - Lymphopenia/*immunology
MH  - Middle Aged
MH  - *Plasmids
MH  - Platelet Endothelial Cell Adhesion Molecule-1/immunology/*metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - T-Lymphocyte Subsets/cytology/*immunology/metabolism
MH  - Telomerase/metabolism
MH  - Telomere/metabolism
MH  - Thymus Gland/cytology/growth & development
EDAT- 2007/10/16 09:00
MHDA- 2007/12/27 09:00
CRDT- 2007/10/16 09:00
PHST- 2007/10/16 09:00 [pubmed]
PHST- 2007/12/27 09:00 [medline]
PHST- 2007/10/16 09:00 [entrez]
AID - 10.1002/eji.200636976 [doi]
PST - ppublish
SO  - Eur J Immunol. 2007 Nov;37(11):3270-80. doi: 10.1002/eji.200636976.