PMID- 17935627
OWN - NLM
STAT- MEDLINE
DCOM- 20080104
LR  - 20211020
IS  - 1471-213X (Electronic)
IS  - 1471-213X (Linking)
VI  - 7
DP  - 2007 Oct 12
TI  - Sex-specific DoublesexM expression in subsets of Drosophila somatic gonad cells.
PG  - 113
AB  - BACKGROUND: In Drosophila melanogaster, a pre-mRNA splicing hierarchy controls 
      sexual identity and ultimately leads to sex-specific Doublesex (DSX) 
      transcription factor isoforms. The male-specific DSXM represses genes involved in 
      female development and activates genes involved in male development. Spatial and 
      temporal control of dsx during embryogenesis is not well documented. RESULTS: 
      Here we show that DSX(M) is specifically expressed in subsets of male somatic 
      gonad cells during embryogenesis. Following testis formation, germ cells remain 
      in contact with DSX(M)-expressing cells, including hub cells and premeiotic 
      somatic cyst cells that surround germ cells during spermatogenesis in larval and 
      adult testes. CONCLUSION: We show that dsx is transcriptionally regulated in 
      addition to being regulated at the pre-mRNA splicing level by the sex 
      determination hierarchy. The dsx locus is spatially controlled by somatic gonad 
      identity. The continuous expression of DSX(M) in cells contacting the germline 
      suggests an ongoing short-range influence of the somatic sex determination 
      pathway on germ cell development.
FAU - Hempel, Leonie U
AU  - Hempel LU
AD  - Laboratory of Cellular and Developmental Biology, NIDDK, National Institutes of 
      Health, 50 South Drive, Bethesda MD 20892 USA. hempell@niddk.nih.gov
FAU - Oliver, Brian
AU  - Oliver B
LA  - eng
GR  - Z01 DK015600-12/ImNIH/Intramural NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Intramural
DEP - 20071012
PL  - England
TA  - BMC Dev Biol
JT  - BMC developmental biology
JID - 100966973
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (DSX protein, Drosophila)
RN  - 0 (Drosophila Proteins)
RN  - 0 (RNA Precursors)
SB  - IM
MH  - Alternative Splicing
MH  - Animals
MH  - DNA-Binding Proteins/*genetics
MH  - Drosophila Proteins/*genetics
MH  - Drosophila melanogaster/embryology/*genetics
MH  - Female
MH  - Fluorescent Antibody Technique
MH  - *Gene Expression Regulation, Developmental
MH  - *Genes, Insect
MH  - Male
MH  - RNA Precursors/genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - *Sex Determination Processes
MH  - Spermatogenesis
MH  - Testis/embryology
PMC - PMC2148063
EDAT- 2007/10/16 09:00
MHDA- 2008/01/05 09:00
PMCR- 2007/10/12
CRDT- 2007/10/16 09:00
PHST- 2007/04/10 00:00 [received]
PHST- 2007/10/12 00:00 [accepted]
PHST- 2007/10/16 09:00 [pubmed]
PHST- 2008/01/05 09:00 [medline]
PHST- 2007/10/16 09:00 [entrez]
PHST- 2007/10/12 00:00 [pmc-release]
AID - 1471-213X-7-113 [pii]
AID - 10.1186/1471-213X-7-113 [doi]
PST - epublish
SO  - BMC Dev Biol. 2007 Oct 12;7:113. doi: 10.1186/1471-213X-7-113.