PMID- 17935670
OWN - NLM
STAT- MEDLINE
DCOM- 20071129
LR  - 20181201
IS  - 0366-6999 (Print)
IS  - 0366-6999 (Linking)
VI  - 120
IP  - 19
DP  - 2007 Oct 5
TI  - Upregulated functional expression of Toll like receptor 4 in mesenchymal stem 
      cells induced by lipopolysaccharide.
PG  - 1685-8
AB  - BACKGROUND: The coordinated change of haematopoietic supporting microenvironment 
      in bone marrow (BM) is crucial for innate immunity and inflammation. As the 
      precursors of marrow stroma, BM derived mesenchymal stem cells (MSCs) promote 
      haematopoietic function, but their roles in innate immunity or inflammation have 
      not been investigated. Here we investigated the expression of Toll like receptor 
      4 (TLR-4) and the effect of lipopolysaccharide (LPS) on its expression in BM MSCs 
      in vitro. METHODS: MSCs were harvested from adult rat's BM cells by density 
      gradient centrifugation and adhesive culture. The purity of MSCs were identified 
      with the cell morphological feature and osteogenic capacity, the phenotypes were 
      tested by flow cytometry. Cultured MSCs were treated by LPS (1 microg/ml, 10 
      microg/ml or 100 microg/ml) for 24 hours. The relative expression levels of TLR-4 
      mRNA were detected by semiquantitative reverse transcription polymerase chain 
      reaction and costimulatory molecules (CD80, CD86 and MHC-II) expressed on MSCs 
      were analyzed by flow cytometry. The levels of tumor necrosis factor-alpha 
      (TNF-alpha) in supernatants were determined by enzyme linked immunosorbent assay. 
      RESULTS: After incubation with LPS, MSCs expressed the higher levels of TLR-4 
      mRNA, costimulatory molecules and TNF-alpha than the untreated group: LPS 10 
      microg/ml was the most effective (P < 0.01); the levels of TLR-4 mRNA, 
      costimulatory molecules and TNF-alpha decreased when MSCs were exposed to 100 
      microg/ml LPS. Except for MHC-II and TNF-alpha (P > 0.05), the levels of CD80, 
      CD86 and TLR-4 mRNA were significantly lower than that in the treated group of 10 
      microg/ml (P < 0.01). CONCLUSION: MSCs expressed TLR-4 mRNA. LPS activated the 
      functional expression levels of TLR-4 in MSCs although the activity may depend on 
      the concentration of LPS.
FAU - Shi, Liang
AU  - Shi L
AD  - Department of Hematology, Affiliated Hospital of Guiyang Medical College, Guiyang 
      550004, China.
FAU - Wang, Ji-shi
AU  - Wang JS
FAU - Liu, Xing-mei
AU  - Liu XM
FAU - Hu, Xiao-yan
AU  - Hu XY
FAU - Fang, Qin
AU  - Fang Q
LA  - eng
PT  - Journal Article
PL  - China
TA  - Chin Med J (Engl)
JT  - Chinese medical journal
JID - 7513795
RN  - 0 (B7-2 Antigen)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Tlr4 protein, rat)
RN  - 0 (Toll-Like Receptor 4)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Animals
MH  - B7-2 Antigen/analysis
MH  - Bone Marrow Cells/immunology
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - Immunophenotyping
MH  - Lipopolysaccharides/*pharmacology
MH  - Male
MH  - Mesenchymal Stem Cells/drug effects/*immunology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Toll-Like Receptor 4/*physiology
MH  - Tumor Necrosis Factor-alpha/biosynthesis
MH  - Up-Regulation
EDAT- 2007/10/16 09:00
MHDA- 2007/12/06 09:00
CRDT- 2007/10/16 09:00
PHST- 2007/10/16 09:00 [pubmed]
PHST- 2007/12/06 09:00 [medline]
PHST- 2007/10/16 09:00 [entrez]
PST - ppublish
SO  - Chin Med J (Engl). 2007 Oct 5;120(19):1685-8.