PMID- 17936239
OWN - NLM
STAT- MEDLINE
DCOM- 20080306
LR  - 20191210
IS  - 0003-2697 (Print)
IS  - 0003-2697 (Linking)
VI  - 373
IP  - 2
DP  - 2008 Feb 15
TI  - Block copolymer-oligonucleotide conjugates for genotyping on microarrays.
PG  - 229-38
AB  - Polymer-oligonucleotide conjugates were synthesized from the amphiphilic block 
      copolymer 
      poly(tert-butylacrylamide-b-(N-acryloylmorpholine-co-N-acryloxysuccinimide)) 
      using an original solid-phase DNA synthesis strategy. This method provided 
      conjugates highly functionalized with oligonucleotides throughout the polymer 
      chain. After purification, block copolymer-oligonucleotide conjugates were 
      spotted on a multidetection microarray system developed by Apibio using a 
      standard nanodroplet piezo inkjet spotting technique to develop the oligosorbent 
      assay (OLISA). Two genotyping models (HLA-DQB1 and platelet glycoproteins [GPs]), 
      which are particularly difficult to study with standard systems, were evaluated. 
      For both models, block copolymer-oligonucleotide conjugates used as capture 
      probes amplified the responses of in vitro diagnostic assays. The detection limit 
      reached by using conjugates was estimated at 15 pM for a 219-bp DNA target 
      (HLA-DQB1 model). Moreover, single nucleotide polymorphism was detected in the 
      platelet GPs genotyping model. The use of polymer conjugates led to a significant 
      improvement in both sensitivity and specificity of standard hybridization assays 
      even when applied to complex biological models.
FAU - de Lambert, Bertrand
AU  - de Lambert B
AD  - Unite Mixte CNRS/bioMerieux, IFR 128 Biosciences Lyon Gerland, Ecole Normale 
      Superieure de Lyon, 69364 Lyon Cedex 07, France.
FAU - Chaix, Carole
AU  - Chaix C
FAU - Charreyre, Marie-Therese
AU  - Charreyre MT
FAU - Martin, Thibault
AU  - Martin T
FAU - Aigoui, Arnaud
AU  - Aigoui A
FAU - Perrin-Rubens, Agnes
AU  - Perrin-Rubens A
FAU - Pichot, Christian
AU  - Pichot C
FAU - Mandrand, Bernard
AU  - Mandrand B
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070912
PL  - United States
TA  - Anal Biochem
JT  - Analytical biochemistry
JID - 0370535
RN  - 0 (Acrylic Resins)
RN  - 0 (HLA-DQ Antigens)
RN  - 0 (HLA-DQ beta-Chains)
RN  - 0 (HLA-DQB1 antigen)
RN  - 0 (Oligonucleotides)
RN  - 0 (Platelet Membrane Glycoproteins)
RN  - 0 (poly(tert-butylacrylamide-b-(N-acryloylmorpholine-co-N-acryloxysuccinimide)))
SB  - IM
MH  - Acrylic Resins/*chemistry
MH  - Genotype
MH  - HLA-DQ Antigens/*genetics
MH  - HLA-DQ beta-Chains
MH  - Humans
MH  - Oligonucleotide Array Sequence Analysis/*instrumentation/methods
MH  - Oligonucleotides/*chemistry
MH  - Platelet Membrane Glycoproteins/*genetics
MH  - Polymorphism, Single Nucleotide
MH  - Spectrometry, Fluorescence
EDAT- 2007/10/16 09:00
MHDA- 2008/03/07 09:00
CRDT- 2007/10/16 09:00
PHST- 2007/07/26 00:00 [received]
PHST- 2007/09/05 00:00 [revised]
PHST- 2007/09/05 00:00 [accepted]
PHST- 2007/10/16 09:00 [pubmed]
PHST- 2008/03/07 09:00 [medline]
PHST- 2007/10/16 09:00 [entrez]
AID - S0003-2697(07)00597-0 [pii]
AID - 10.1016/j.ab.2007.09.008 [doi]
PST - ppublish
SO  - Anal Biochem. 2008 Feb 15;373(2):229-38. doi: 10.1016/j.ab.2007.09.008. Epub 2007 
      Sep 12.