PMID- 17938328
OWN - NLM
STAT- MEDLINE
DCOM- 20071101
LR  - 20220331
IS  - 0886-4470 (Print)
IS  - 0886-4470 (Linking)
VI  - 133
IP  - 10
DP  - 2007 Oct
TI  - Amplification and overexpression of HER2/neu gene and HER2/neu protein in 
      salivary duct carcinoma of the parotid gland.
PG  - 1031-6
AB  - OBJECTIVES: To detect amplification of the HER2/neu gene by means of fluorescence 
      in situ hybridization (FISH) in a series of 13 salivary duct carcinomas (SDCs) 
      and to compare the results with immunohistochemical (IHC) assessment of HER2/neu 
      protein expression. DESIGN: Retrospective analysis. SETTING: Department of 
      Pathology, University of Brescia. PATIENTS: We studied 13 cases of SDC diagnosed 
      between January 1, 1997, and June 30, 2004, all arising from the parotid gland. 
      Twelve patients were treated with surgery and radiotherapy, and 1 patient 
      received only palliative radiotherapy. Seven patients died of disease, 3 patients 
      were alive with disease, and 3 were free of disease. MAIN OUTCOME MEASURES: 
      HER2/neu protein expression and HER2/neu gene amplification detected by means of 
      IHC assessment and FISH, respectively. RESULTS: With IHC assessment, 10 cases 
      showed overexpression (grade 3+) of HER2/neu protein, whereas 3 cases were 
      negative for this protein (grade 0/1+). Using FISH, amplification of the HER2/neu 
      gene was found in 8 of the 10 grade 3+ cases, whereas none of the cases negative 
      for the protein according to IHC assessment had amplification of the gene. 
      Because of the small number of patients, it was not possible to statistically 
      correlate HER2/neu protein expression or HER2/neu gene amplification and 
      survival. CONCLUSION: Our data demonstrate that HER2/neu protein is frequently 
      overexpressed in SDC, and in contrast to previous reports, overexpression of the 
      protein is associated in most cases with HER2/neu gene amplification.
FAU - Cornolti, Giorgio
AU  - Cornolti G
AD  - Department of Pathology, University of Brescia, Piazza Spedali Civili 1, 25100 
      Brescia, Italy.
FAU - Ungari, Marco
AU  - Ungari M
FAU - Morassi, Maria Laura
AU  - Morassi ML
FAU - Facchetti, Fabio
AU  - Facchetti F
FAU - Rossi, Elisa
AU  - Rossi E
FAU - Lombardi, Davide
AU  - Lombardi D
FAU - Nicolai, Piero
AU  - Nicolai P
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Arch Otolaryngol Head Neck Surg
JT  - Archives of otolaryngology--head & neck surgery
JID - 8603209
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (DNA, Neoplasm)
RN  - EC 2.7.10.1 (Receptor, ErbB-2)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Biomarkers, Tumor/genetics/metabolism
MH  - Carcinoma, Ductal/*genetics/metabolism/pathology
MH  - DNA, Neoplasm/*genetics
MH  - Diagnosis, Differential
MH  - Female
MH  - *Gene Amplification
MH  - *Gene Expression Regulation, Neoplastic
MH  - Genes, erbB-2/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - Nucleic Acid Amplification Techniques
MH  - Parotid Neoplasms/*genetics/metabolism/pathology
MH  - Prognosis
MH  - Receptor, ErbB-2/biosynthesis/*genetics
MH  - Retrospective Studies
EDAT- 2007/10/17 09:00
MHDA- 2007/11/02 09:00
CRDT- 2007/10/17 09:00
PHST- 2007/10/17 09:00 [pubmed]
PHST- 2007/11/02 09:00 [medline]
PHST- 2007/10/17 09:00 [entrez]
AID - 133/10/1031 [pii]
AID - 10.1001/archotol.133.10.1031 [doi]
PST - ppublish
SO  - Arch Otolaryngol Head Neck Surg. 2007 Oct;133(10):1031-6. doi: 
      10.1001/archotol.133.10.1031.