PMID- 17939401
OWN - NLM
STAT- MEDLINE
DCOM- 20090930
LR  - 20071017
IS  - 0253-2727 (Print)
IS  - 0253-2727 (Linking)
VI  - 28
IP  - 6
DP  - 2007 Jun
TI  - [Human multiple myeloma cells stimulate differentiation of endothelial cells to 
      form capillary-like networks in two different co-culture systems].
PG  - 375-8
AB  - OBJECTIVE: To study the influence of multiple myeloma cells on differentiation of 
      endothelial cells to form capillary-like networks and the role of brain derived 
      neurotrophic factor (BDNF) in this process. METHODS: Human multiple myeloma cell 
      line RPMI8226 or fresh myeloma cells were co-cultured with human umbilical vein 
      endothelial cells (HUVECs) in two different systems: the contact and the 
      non-contact systems. The HUVECs cultured alone were used as control. The effects 
      of soluble cytokine and adhesion molecule on angiogenesis in HUVECs co-cultured 
      with MM cell were studied by modified Matrigel capillary-like networks formation 
      assay and BDNF levels in co-culture system supernatant by enzyme-linked 
      immunosorbent assay. In the contact co-culture system, the formation of 
      capillary-like networks and the secretion of BDNF were detected again after MM 
      cell was preincubated and cultured with anti-CD29 and anti-CD18 monoclonal 
      antibodies. RESULTS: In RPMI8226 co-culture system, the number of capillary-like 
      structure was increased in non-contact HUVECs system compared with that in 
      monoculture, but the increase was lower than that of contact HUVECs system (75% 
      vs. 113%). In fresh myeloma cells co-culture system, the numbers of 
      capillary-like structure were 138% and 188% for the non-contact and the contact 
      co-culture systems, respectively, above that in HUVECs cultured alone. The median 
      BDNF concentrations in culture supernatants of mono-cultured, contact co-cultured 
      with RPMI8226, non-contact co-cultured with RPMI8226, contact co-cultured with 
      fresh myeloma cells and non-contact co-cultured with fresh myeloma cells were 
      (12.4 +/- 5.1) ng/ ml, (38.5 +/- 8.2) ng/ml, (31.6 +/- 7.2) ng/ml, (37.1 +/- 8.7) 
      ng/ml and(27.9 +/- 7.6) ng/ml, respectively. Either of the two adhesion molecule 
      monoclonal antibodies reduced the capillary-like networks formation and the BDNF 
      secretion in the contact co-culture system. CONCLUSION: Human multiple myeloma 
      cells stimulate differentiation of endothelial cells to form capillary-like 
      networks in two different co-culture systems. BDNF takes part in this progress 
      and is modulated by soluble cytokine and adhesion molecule expressed by both 
      kinds of cells.
FAU - Wang, Ya-dan
AU  - Wang YD
AD  - Institution of Hematology, Affiliated Union Hospital Tongji Medical College, 
      Huazhong University of Science and Technology, Wuhan 430022, China.
FAU - Hu, Yu
AU  - Hu Y
FAU - Sun, Chun-yan
AU  - Sun CY
FAU - He, Wen-juan
AU  - He WJ
FAU - Zhang, Xiao-ping
AU  - Zhang XP
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Xue Ye Xue Za Zhi
JT  - Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi
JID - 8212398
RN  - 0 (Brain-Derived Neurotrophic Factor)
SB  - IM
MH  - Brain-Derived Neurotrophic Factor/metabolism
MH  - Cell Adhesion
MH  - *Cell Differentiation
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Endothelial Cells/*cytology/metabolism
MH  - Endothelium, Vascular/cytology
MH  - Humans
MH  - Multiple Myeloma/*pathology
MH  - Neovascularization, Pathologic
MH  - Umbilical Veins/cytology
EDAT- 2007/10/18 09:00
MHDA- 2009/10/01 06:00
CRDT- 2007/10/18 09:00
PHST- 2007/10/18 09:00 [pubmed]
PHST- 2009/10/01 06:00 [medline]
PHST- 2007/10/18 09:00 [entrez]
PST - ppublish
SO  - Zhonghua Xue Ye Xue Za Zhi. 2007 Jun;28(6):375-8.