PMID- 17939949
OWN - NLM
STAT- MEDLINE
DCOM- 20080611
LR  - 20171116
IS  - 0898-6568 (Print)
IS  - 0898-6568 (Linking)
VI  - 20
IP  - 1
DP  - 2008 Jan
TI  - Toll-like receptor 9-stimulated monocyte chemoattractant protein-1 is mediated 
      via JNK-cytosolic phospholipase A2-ROS signaling.
PG  - 105-11
AB  - Monocyte chemoattractant protein-1 (MCP-1) influences monocyte migration into 
      sites of inflammation. This study highlights the importance of cytosolic 
      phospholipase A2 (cPLA2)-mediated reactive oxygen species (ROS) signaling 
      processes in the regulation of MCP-1 release as a result of toll-like receptor 
      (TLR) activation. In macrophages, activation of TLR9 induced MCP-1 and 
      cPLA2-phosphorylated arachidonic acid (AA) release. Inhibition of cPLA2 blocked 
      CpG-induced MCP-1 and AA release. Although CpG stimulates phosphorylation of ERK, 
      p38 and JNK, only inhibition of the JNK signaling pathways attenuated MCP-1 
      release, suggesting that the TLR9-mediated MCP-1 release was dependent upon the 
      JNK pathway. TLR9 activation also stimulated ROS generation, while inhibition of 
      NADPH oxidases (Noxs) blocked CpG-induced MCP-1 release. The CpG treatment 
      increased macrophage Nox1 mRNA level, however it had no effect on macrophage Nox2 
      mRNA level. Overall, these results suggest that CpG enhances ROS generation 
      through cPLA2-dependent pathways, which results in MCP-1 release.
FAU - Lee, Jin-Gu
AU  - Lee JG
AD  - Aging-associated Vascular Disease Research Center, Department of Biochemistry & 
      Molecular Biology, College of Medicine, Yeungnam University, 317-1 Daemyung-5 
      Dong, Nam-Gu, Daegu 705-717, South Korea.
FAU - Lee, Sun-Hye
AU  - Lee SH
FAU - Park, Dae-Weon
AU  - Park DW
FAU - Lee, Sang-Hoon
AU  - Lee SH
FAU - Yoon, Hong-Sik
AU  - Yoon HS
FAU - Chin, Byung-Ro
AU  - Chin BR
FAU - Kim, Jung-Hye
AU  - Kim JH
FAU - Kim, Jae-Ryong
AU  - Kim JR
FAU - Baek, Suk-Hwan
AU  - Baek SH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070922
PL  - England
TA  - Cell Signal
JT  - Cellular signalling
JID - 8904683
RN  - 0 (Amino Acids)
RN  - 0 (CPG-oligonucleotide)
RN  - 0 (Ccl2 protein, mouse)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Oligodeoxyribonucleotides)
RN  - 0 (RNA, Messenger)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Tlr9 protein, mouse)
RN  - 0 (Toll-Like Receptor 9)
RN  - EC 1.6.- (NADH, NADPH Oxidoreductases)
RN  - EC 1.6.3.- (Cybb protein, mouse)
RN  - EC 1.6.3.- (NADPH Oxidase 1)
RN  - EC 1.6.3.- (NADPH Oxidase 2)
RN  - EC 1.6.3.- (NADPH Oxidases)
RN  - EC 1.6.3.- (NOX1 protein, mouse)
RN  - EC 2.7.12.2 (MAP Kinase Kinase 4)
RN  - EC 3.1.1.4 (Phospholipases A2, Cytosolic)
SB  - IM
MH  - Amino Acids/metabolism
MH  - Animals
MH  - Cell Line
MH  - Chemokine CCL2/*metabolism
MH  - MAP Kinase Kinase 4/*metabolism
MH  - Macrophages/cytology/drug effects/*metabolism
MH  - Membrane Glycoproteins/metabolism
MH  - Mice
MH  - NADH, NADPH Oxidoreductases/metabolism
MH  - NADPH Oxidase 1
MH  - NADPH Oxidase 2
MH  - NADPH Oxidases/metabolism
MH  - Oligodeoxyribonucleotides/pharmacology
MH  - Phospholipases A2, Cytosolic/*metabolism
MH  - RNA, Messenger/metabolism
MH  - Reactive Oxygen Species/*metabolism
MH  - Signal Transduction/*physiology
MH  - Toll-Like Receptor 9/*metabolism
EDAT- 2007/10/18 09:00
MHDA- 2008/06/12 09:00
CRDT- 2007/10/18 09:00
PHST- 2007/07/04 00:00 [received]
PHST- 2007/09/17 00:00 [revised]
PHST- 2007/09/17 00:00 [accepted]
PHST- 2007/10/18 09:00 [pubmed]
PHST- 2008/06/12 09:00 [medline]
PHST- 2007/10/18 09:00 [entrez]
AID - S0898-6568(07)00287-2 [pii]
AID - 10.1016/j.cellsig.2007.09.003 [doi]
PST - ppublish
SO  - Cell Signal. 2008 Jan;20(1):105-11. doi: 10.1016/j.cellsig.2007.09.003. Epub 2007 
      Sep 22.