PMID- 17940612
OWN - NLM
STAT- MEDLINE
DCOM- 20080715
LR  - 20211027
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 2
IP  - 10
DP  - 2007 Oct 17
TI  - Murine gammaherpesvirus-68 inhibits antigen presentation by dendritic cells.
PG  - e1048
LID - e1048
AB  - Dendritic cells (DCs) play a central role in initiating adaptive immunity. Murine 
      gammaherpesvirus-68 (MHV-68), like many persistent viruses, infects DCs during 
      normal host colonization. It therefore provides a means to understanding what 
      host and viral genes contribute to this aspect of pathogenesis. The infected DC 
      phenotype is likely to depend on whether viral gene expression is lytic or latent 
      and whether antigen presentation is maintained. For MHV-68, neither parameter has 
      been well defined. Here we show that MHV-68 infects immature but not mature bone 
      marrow-derived DCs. Infection was predominantly latent and these DCs showed no 
      obvious defect in antigen presentation. Lytically infected DCs were very 
      different. These down-regulated CD86 and MHC class I expression and presented a 
      viral epitope poorly to CD8(+) T cells. Antigen presentation improved markedly 
      when the MHV-68 K3 gene was disrupted, indicating that K3 fulfils an important 
      function in infected DCs. MHV-68 infects only a small fraction of the DCs present 
      in lymphoid tissue, so K3 expression is unlikely to compromise significantly 
      global CD8(+) T cell priming. Instead it probably helps to maintain lytic gene 
      expression in DCs once CD8(+) T cell priming has occurred.
FAU - Smith, Christopher M
AU  - Smith CM
AD  - Division of Virology, Department of Pathology, University of Cambridge, 
      Cambridge, United Kingdom.
FAU - Gill, Michael B
AU  - Gill MB
FAU - May, Janet S
AU  - May JS
FAU - Stevenson, Philip G
AU  - Stevenson PG
LA  - eng
GR  - G0400427/MRC_/Medical Research Council/United Kingdom
GR  - C19612/A6189/CRUK_/Cancer Research UK/United Kingdom
GR  - G9800903/MRC_/Medical Research Council/United Kingdom
GR  - GR076956MA/WT_/Wellcome Trust/United Kingdom
GR  - WT_/Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20071017
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Epitopes)
RN  - 0 (Histocompatibility Antigens Class I)
SB  - IM
MH  - Animals
MH  - *Antigen Presentation
MH  - CD8-Positive T-Lymphocytes/metabolism
MH  - Cricetinae
MH  - Dendritic Cells/*virology
MH  - Epitopes/chemistry
MH  - Gammaherpesvirinae/*metabolism
MH  - *Gene Expression Regulation
MH  - Histocompatibility Antigens Class I
MH  - Lymphocyte Activation
MH  - Lymphoid Tissue/metabolism
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - NIH 3T3 Cells
PMC - PMC2002512
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2007/10/18 09:00
MHDA- 2008/07/17 09:00
PMCR- 2007/10/17
CRDT- 2007/10/18 09:00
PHST- 2007/08/20 00:00 [received]
PHST- 2007/10/01 00:00 [accepted]
PHST- 2007/10/18 09:00 [pubmed]
PHST- 2008/07/17 09:00 [medline]
PHST- 2007/10/18 09:00 [entrez]
PHST- 2007/10/17 00:00 [pmc-release]
AID - 07-PONE-RA-02026R1 [pii]
AID - 10.1371/journal.pone.0001048 [doi]
PST - epublish
SO  - PLoS One. 2007 Oct 17;2(10):e1048. doi: 10.1371/journal.pone.0001048.