PMID- 17941910
OWN - NLM
STAT- MEDLINE
DCOM- 20081006
LR  - 20181201
IS  - 1365-2222 (Electronic)
IS  - 0954-7894 (Linking)
VI  - 37
IP  - 12
DP  - 2007 Dec
TI  - Can hypertonic saline inhalation influence preformed chemokine and mediator 
      release in induced sputum of chronic obstructive pulmonary disease patients? 
      Comparison with isotonic saline.
PG  - 1819-26
AB  - BACKGROUND: Hypertonic saline (HS) has been shown to modulate in vitro cell 
      functions according to the state of cell activation; however, few studies have 
      evaluated the effect of HS in vivo. Chronic airway inflammation, a major feature 
      of chronic obstructive pulmonary disease (COPD), is associated with an activation 
      of inflammatory and resident cells, which in turn makes them more prompt to 
      respond to further stimuli. OBJECTIVE: To evaluate whether HS might modulate, 
      also in vivo, the release of preformed mediators and intracellular chemokines 
      from airway cells of COPD patients. METHODS: Sputum was induced by inhalation of 
      either HS (4.5% w/v) or isotonic saline (IS 0.9% w/v) solution and processed by 
      plug selection. We measured eosinophil cationic protein (ECP), neutrophil 
      elastase (NE), IL-8 and monocyte chemoattractant protein-1 (MCP-1) in sputum 
      samples obtained by either HS or IS inhalation in 24 COPD patients. RESULTS: No 
      significant difference in mediators measured in sputum samples obtained by the 
      two different inductions was observed; also, there was no significant difference 
      in sputum sample volumes, cell viability, total and differential cell counts. 
      Repeatability between the two tests was high for ECP, NE, macrophages, 
      neutrophils and eosinophils, and satisfactory for IL-8 and MCP-1. CONCLUSIONS: 
      Hyperosmolarity does not affect the levels of the inflammatory mediators and 
      chemokines examined or the cell counts measured in induced sputum obtained from 
      COPD patients. This study does not support the hypothesis that HS can stimulate 
      chemokine and mediator release from airway cells of COPD patients. Therefore, HS 
      and IS can be interchangeably used to measure inflammatory mediators in the 
      sputum supernatant of COPD patients.
FAU - Cianchetti, S
AU  - Cianchetti S
AD  - CardioThoracic Department, Pneumology Section, Pisa, Italy. 
      s.cianchetti@dcap.med.unipi.it
FAU - Bacci, E
AU  - Bacci E
FAU - Bartoli, M L
AU  - Bartoli ML
FAU - Ruocco, L
AU  - Ruocco L
FAU - Pavia, T
AU  - Pavia T
FAU - Dente, F L
AU  - Dente FL
FAU - Di Franco, A
AU  - Di Franco A
FAU - Vagaggini, B
AU  - Vagaggini B
FAU - Paggiaro, P
AU  - Paggiaro P
LA  - eng
PT  - Clinical Trial
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20071017
PL  - England
TA  - Clin Exp Allergy
JT  - Clinical and experimental allergy : journal of the British Society for Allergy 
      and Clinical Immunology
JID - 8906443
RN  - 0 (Chemokines)
RN  - 0 (Isotonic Solutions)
RN  - 0 (Saline Solution, Hypertonic)
SB  - IM
MH  - Aged
MH  - Chemokines/biosynthesis/*metabolism
MH  - Female
MH  - Humans
MH  - Inhalation
MH  - Isotonic Solutions/administration & dosage/*pharmacology
MH  - Male
MH  - Pulmonary Disease, Chronic Obstructive/*metabolism/physiopathology
MH  - Saline Solution, Hypertonic/administration & dosage/*pharmacology
MH  - Solubility
MH  - Sputum/*drug effects/*metabolism
EDAT- 2007/10/19 09:00
MHDA- 2008/10/07 09:00
CRDT- 2007/10/19 09:00
PHST- 2007/10/19 09:00 [pubmed]
PHST- 2008/10/07 09:00 [medline]
PHST- 2007/10/19 09:00 [entrez]
AID - CEA2850 [pii]
AID - 10.1111/j.1365-2222.2007.02850.x [doi]
PST - ppublish
SO  - Clin Exp Allergy. 2007 Dec;37(12):1819-26. doi: 10.1111/j.1365-2222.2007.02850.x. 
      Epub 2007 Oct 17.