PMID- 17942805
OWN - NLM
STAT- MEDLINE
DCOM- 20080130
LR  - 20161124
IS  - 0161-5505 (Print)
IS  - 0161-5505 (Linking)
VI  - 48
IP  - 11
DP  - 2007 Nov
TI  - Radiolabeled Monocyte Chemotactic Protein 1 for the detection of inflammation in 
      experimental atherosclerosis.
PG  - 1816-21
AB  - Chemotactic peptides, such as Monocyte Chemotactic Protein 1 (MCP-1), play a key 
      role in transendothelial migration of mononuclear cells during the development 
      and progression of atherosclerotic disease. Because atherosclerotic plaques that 
      are precursors of acute coronary events harbor abundant macrophage infiltration, 
      we hypothesized that the detection of a high concentration of MCP-1 receptors on 
      inflammatory cells should noninvasively identify vulnerable plaques. METHODS: 
      Atherosclerotic lesions were induced by balloon deendothelialization of the 
      abdominal aorta, which was followed by a 0.5% cholesterol diet for 16 wk in 7 New 
      Zealand White rabbits; 5 unmanipulated rabbits, fed normal chow for 16 wk, were 
      used as controls. Radionuclide imaging was performed immediately after 
      intravenous (99m)Tc-labeled MCP-1 administration and 3 h later. At the end of 
      imaging session, aortas were explanted and submitted for estimation of 
      quantitative MCP-1 uptake (in percentage injected dose per gram, %ID/g) and 
      pathologic characterization. RESULTS: Atherosclerotic lesions were clearly 
      visible in all hyperlipidemic animal gamma-imaging. No tracer uptake was seen in 
      the control rabbits. The mean quantitative MCP-1 uptake in atherosclerotic 
      lesions was 4-fold higher than that of the aortic specimens from the control 
      rabbits (0.065 +/- 0.005 vs. 0.016 +/- 0.006; P < 0.0001). Histology confirmed a 
      strong correlation between MCP-1 uptake and the number of macrophages in American 
      Heart Association type II-IV lesions (r = 0.87, P < 0.0001). CONCLUSION: 
      Noninvasive radionuclide imaging of inflammation is feasible by MCP-1 in 
      experimentally induced atherosclerosis. It is proposed that detection of the 
      extent of inflammation in advanced atherosclerotic plaques may allow 
      identification of unstable plaques.
FAU - Hartung, Dagmar
AU  - Hartung D
AD  - University of California School of Medicine, Irvine, CA 92697, USA.
FAU - Petrov, Artiom
AU  - Petrov A
FAU - Haider, Nezam
AU  - Haider N
FAU - Fujimoto, Shinichiro
AU  - Fujimoto S
FAU - Blankenberg, Francis
AU  - Blankenberg F
FAU - Fujimoto, Ai
AU  - Fujimoto A
FAU - Virmani, Renu
AU  - Virmani R
FAU - Kolodgie, Frank D
AU  - Kolodgie FD
FAU - Strauss, H William
AU  - Strauss HW
FAU - Narula, Jagat
AU  - Narula J
LA  - eng
GR  - R01 HL078681/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20071017
PL  - United States
TA  - J Nucl Med
JT  - Journal of nuclear medicine : official publication, Society of Nuclear Medicine
JID - 0217410
RN  - 0 (Chemokine CCL2)
RN  - 0 (Radiopharmaceuticals)
RN  - 0 (Recombinant Proteins)
RN  - A0730CX801 (Sodium Pertechnetate Tc 99m)
SB  - IM
MH  - Animals
MH  - Atherosclerosis/*diagnostic imaging/immunology/pathology
MH  - Chemokine CCL2/*metabolism
MH  - Diet, Atherogenic
MH  - Humans
MH  - Inflammation/diagnosis/immunology/metabolism
MH  - Macrophages/metabolism
MH  - Rabbits
MH  - Radionuclide Imaging
MH  - Radiopharmaceuticals
MH  - Recombinant Proteins
MH  - Sodium Pertechnetate Tc 99m
EDAT- 2007/10/19 09:00
MHDA- 2008/01/31 09:00
CRDT- 2007/10/19 09:00
PHST- 2007/10/19 09:00 [pubmed]
PHST- 2008/01/31 09:00 [medline]
PHST- 2007/10/19 09:00 [entrez]
AID - jnumed.107.043463 [pii]
AID - 10.2967/jnumed.107.043463 [doi]
PST - ppublish
SO  - J Nucl Med. 2007 Nov;48(11):1816-21. doi: 10.2967/jnumed.107.043463. Epub 2007 
      Oct 17.