PMID- 17947375
OWN - NLM
STAT- MEDLINE
DCOM- 20080709
LR  - 20211020
IS  - 0888-8809 (Print)
IS  - 1944-9917 (Electronic)
IS  - 0888-8809 (Linking)
VI  - 22
IP  - 2
DP  - 2008 Feb
TI  - SH2B1beta (SH2-Bbeta) enhances expression of a subset of nerve growth 
      factor-regulated genes important for neuronal differentiation including genes 
      encoding urokinase plasminogen activator receptor and matrix metalloproteinase 
      3/10.
PG  - 454-76
AB  - Previous work showed that the adapter protein SH2B adapter protein 1beta (SH2B1) 
      (SH2-B) binds to the activated form of the nerve growth factor (NGF) receptor 
      TrkA and is critical for both NGF-dependent neurite outgrowth and maintenance. To 
      identify SH2B1beta-regulated genes critical for neurite outgrowth, we performed 
      microarray analysis of control PC12 cells and PC12 cells stably overexpressing 
      SH2B1beta (PC12-SH2B1beta) or the dominant-negative SH2B1beta(R555E) 
      [PC12-SH2B1beta(R555E)]. NGF-induced microarray expression of Plaur and Mmp10 
      genes was greatly enhanced in PC12-SH2B1beta cells, whereas NGF-induced Plaur and 
      Mmp3 expression was substantially depressed in PC12-SH2B1beta(R555E) cells. 
      Plaur, Mmp3, and Mmp10 are among the 12 genes most highly up-regulated after 6 h 
      of NGF. Their protein products [urokinase plasminogen activator receptor (uPAR), 
      matrix metalloproteinase 3 (MMP3), and MMP10] lie in the same pathway of 
      extracellular matrix degradation; uPAR has been shown previously to be critical 
      for NGF-induced neurite outgrowth. Quantitative real-time PCR analysis revealed 
      SH2B1beta enhancement of NGF induction of all three genes and the suppression of 
      NGF induction of all three when endogenous SH2B1 was reduced using short hairpin 
      RNA against SH2B1 and in PC12-SH2B1beta(R555E) cells. NGF-induced levels of uPAR 
      and MMP3/10 and neurite outgrowth through Matrigel (MMP3-dependent) were also 
      increased in PC12-SH2B1beta cells. These results suggest that SH2B1beta 
      stimulates NGF-induced neuronal differentiation at least in part by enhancing 
      expression of a specific subset of NGF-sensitive genes, including Plaur, Mmp3, 
      and/or Mmp10, required for neurite outgrowth.
FAU - Chen, Linyi
AU  - Chen L
AD  - Department of Molecular and Integrative Physiology, The University of Michigan 
      Medical School, Ann Arbor, MI 48109-0622, USA.
FAU - Maures, Travis J
AU  - Maures TJ
FAU - Jin, Hui
AU  - Jin H
FAU - Huo, Jeffrey S
AU  - Huo JS
FAU - Rabbani, Shafaat A
AU  - Rabbani SA
FAU - Schwartz, Jessica
AU  - Schwartz J
FAU - Carter-Su, Christin
AU  - Carter-Su C
LA  - eng
SI  - GEO/GSE4557
GR  - R01 DK054222/DK/NIDDK NIH HHS/United States
GR  - T32 DK071212/DK/NIDDK NIH HHS/United States
GR  - 5P60 DK 20572/DK/NIDDK NIH HHS/United States
GR  - P60 DK020572/DK/NIDDK NIH HHS/United States
GR  - R01 DK 54222/DK/NIDDK NIH HHS/United States
GR  - T32 GM 07315/GM/NIGMS NIH HHS/United States
GR  - T32 DK 071212/DK/NIDDK NIH HHS/United States
GR  - T32 GM007315/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20071018
PL  - United States
TA  - Mol Endocrinol
JT  - Molecular endocrinology (Baltimore, Md.)
JID - 8801431
RN  - 0 (Carrier Proteins)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Plaur protein, rat)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Receptors, Urokinase Plasminogen Activator)
RN  - 0 (SH2B1 protein, rat)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
RN  - EC 3.4.24.22 (Matrix Metalloproteinase 10)
SB  - IM
MH  - Animals
MH  - Carrier Proteins/*genetics/metabolism/physiology
MH  - Cell Differentiation/*drug effects/genetics
MH  - Gene Expression Regulation, Neoplastic/drug effects
MH  - Gene Silencing
MH  - Immunoblotting
MH  - Intracellular Signaling Peptides and Proteins
MH  - Matrix Metalloproteinase 10/*genetics/metabolism
MH  - Matrix Metalloproteinase 3/*genetics/metabolism
MH  - Models, Biological
MH  - Nerve Growth Factors/*pharmacology
MH  - Neurites/drug effects/metabolism
MH  - Neurons/cytology/drug effects/metabolism
MH  - Oligonucleotide Array Sequence Analysis
MH  - PC12 Cells
MH  - Rats
MH  - Receptors, Cell Surface/*genetics/metabolism
MH  - Receptors, Urokinase Plasminogen Activator
MH  - Reverse Transcriptase Polymerase Chain Reaction
PMC - PMC2234583
EDAT- 2007/10/20 09:00
MHDA- 2008/07/10 09:00
PMCR- 2009/02/01
CRDT- 2007/10/20 09:00
PHST- 2007/10/20 09:00 [pubmed]
PHST- 2008/07/10 09:00 [medline]
PHST- 2007/10/20 09:00 [entrez]
PHST- 2009/02/01 00:00 [pmc-release]
AID - me.2007-0384 [pii]
AID - 4185 [pii]
AID - 10.1210/me.2007-0384 [doi]
PST - ppublish
SO  - Mol Endocrinol. 2008 Feb;22(2):454-76. doi: 10.1210/me.2007-0384. Epub 2007 Oct 
      18.