PMID- 17948936
OWN - NLM
STAT- MEDLINE
DCOM- 20071129
LR  - 20211020
IS  - 1007-9327 (Print)
IS  - 2219-2840 (Electronic)
IS  - 1007-9327 (Linking)
VI  - 13
IP  - 42
DP  - 2007 Nov 14
TI  - Induction of apoptosis by artemisinin relieving the severity of inflammation in 
      caerulein-induced acute pancreatitis.
PG  - 5612-7
AB  - AIM: To observe the apoptosis and oncosis of pancreatic acinar cells and 
      secondary inflammatory reaction in pancreatic tissue from rats with acute 
      pancreatitis (AP), and the influences of artemisinin on them. METHODS: AP was 
      induced by 4 intraperitoneal injections of caerulein at 1 h intervals. To induce 
      apoptosis, solution of artemisinin (50 mg/kg) was given intraperitoneally 1, 12, 
      24 and 36 h after the last caerulein injection. Histological examination of 
      impairment of pancreatic tissue and detection of serum amylase were performed to 
      evaluate the severity of acute pancreatitis. Apoptosis and oncosis were detected 
      with acridine orange (AO) and ethylene dibromide (EB) staining. Caspase-3 and 
      myeloperoxidase (MPO) activity were measured by colorimetric assay. Nuclear 
      factor-kappa B (NF-kappaB) activation was detected by flow cytometry. Macrophage 
      inflammatory protein-1alpha (MIP-1alpha) protein was measured by Western blot. 
      Interleukin-1beta (IL-1beta) mRNA was detected by RT-PCR. RESULTS: Addition of 
      artemisinin increased the number of apoptotic cells (11.7% +/- 1.4% vs 6.3% +/- 
      0.7%, P < 0.05), while reduced the number of oncotic cells (13.0% +/- 2.4% vs 
      17.5% +/- 2.2%, P < 0.05). The activity of caspase-3 speeded up (1.52 +/- 0.21 vs 
      1.03 +/- 0.08, P < 0.05), the pancreas pathological impairment was relieved (3.0 
      +/- 0.5 vs 4.0 +/- 0.5, P < 0.05) and the level of serum amylase decreased (5642 
      +/- 721 U/dL vs 7821 +/- 653 U/dL, P < 0.05). The activation of NF-kB (29% +/- 
      4.1% vs 42% +/- 5.8%), MIP-1alpha protein (3.7 +/- 0.5 vs 5.8 +/- 0.7), MPO (0.52 
      +/- 0.06 U/g vs 0.68 +/- 0.09 U/g), IL-1beta mRNA (1.7 +/- 0.3 vs 2.4 +/- 0.4) in 
      the apoptosis inducing group was obviously decreased (P < 0.05). CONCLUSION: 
      Inducing apoptosis can relieve pathological impairment and inflammatory reaction 
      in AP rats.
FAU - Zhao, Ming
AU  - Zhao M
AD  - Department of General Surgery, First Clinical College, Harbin Medical University, 
      Harbin 150001, Heilongjiang Province, China.
FAU - Xue, Dong-Bo
AU  - Xue DB
FAU - Zheng, Biao
AU  - Zheng B
FAU - Zhang, Wei-Hui
AU  - Zhang WH
FAU - Pan, Shang-Ha
AU  - Pan SH
FAU - Sun, Bei
AU  - Sun B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - World J Gastroenterol
JT  - World journal of gastroenterology
JID - 100883448
RN  - 0 (Artemisinins)
RN  - 0 (NF-kappa B)
RN  - 888Y08971B (Ceruletide)
RN  - 9RMU91N5K2 (artemisinin)
RN  - EC 1.11.1.7 (Peroxidase)
RN  - EC 3.4.22.- (Caspase 3)
SB  - IM
MH  - Acute Disease
MH  - Animals
MH  - Apoptosis/*drug effects
MH  - Artemisinins/*pharmacology/therapeutic use
MH  - Caspase 3/metabolism
MH  - Ceruletide/toxicity
MH  - Male
MH  - NF-kappa B/metabolism
MH  - Pancreatitis/chemically induced/*drug therapy/metabolism
MH  - Peroxidase/analysis
MH  - Rats
MH  - Rats, Wistar
PMC - PMC4172741
EDAT- 2007/10/24 09:00
MHDA- 2007/12/06 09:00
PMCR- 2007/11/14
CRDT- 2007/10/24 09:00
PHST- 2007/10/24 09:00 [pubmed]
PHST- 2007/12/06 09:00 [medline]
PHST- 2007/10/24 09:00 [entrez]
PHST- 2007/11/14 00:00 [pmc-release]
AID - 10.3748/wjg.v13.i42.5612 [doi]
PST - ppublish
SO  - World J Gastroenterol. 2007 Nov 14;13(42):5612-7. doi: 10.3748/wjg.v13.i42.5612.