PMID- 17949411
OWN - NLM
STAT- MEDLINE
DCOM- 20080205
LR  - 20161124
IS  - 1471-4159 (Electronic)
IS  - 0022-3042 (Linking)
VI  - 104
IP  - 2
DP  - 2008 Jan
TI  - GSK3beta mediates the induced expression of synaptic acetylcholinesterase during 
      apoptosis.
PG  - 409-19
AB  - Besides its role in terminating acetylcholine-mediated neurotransmission, 
      acetylcholinesterase (AChE) is found to be expressed and participate in the 
      process of apoptosis in various cell types. However, the mechanisms underlying 
      AChE up-regulation in neuronal cells remain elusive. Herein we demonstrated that 
      glycogen synthase kinase-3beta (GSK3beta) mediates induced AChE-S expression 
      during apoptosis. In this study, A23187 and thapsigargin (TG) were employed to 
      induce apoptosis in neuroendocrine PC12 cells. The results showed that exposure 
      of PC12 cells to A23187 and TG up-regulated AChE activity significantly. The same 
      treatment also led to activation of GSK3beta. Two different inhibitors of 
      GSK3beta (lithium and GSK3beta-specific inhibitor VIII) could block A23187- or 
      TG-induced up-regulation of AChE activity, AChE-S mRNA level and protein 
      expression. However, lithium could not inhibit the induction of AChE-R mRNA and 
      protein under similar conditions. Taken together, our results show that GSK3beta 
      is specifically involved in the induction of AChE-S expression in PC12 cells 
      during apoptosis.
FAU - Jing, Peng
AU  - Jing P
AD  - Laboratory of Molecular Cell Biology, Institute of Biochemistry and Cell Biology, 
      Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 
      Graduate School of the Chinese Academy of Sciences, Shanghai, China.
FAU - Jin, Qihuang
AU  - Jin Q
FAU - Wu, Jun
AU  - Wu J
FAU - Zhang, Xue-Jun
AU  - Zhang XJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20071018
PL  - England
TA  - J Neurochem
JT  - Journal of neurochemistry
JID - 2985190R
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Iodides)
RN  - 0 (Ionophores)
RN  - 0 (RNA, Messenger)
RN  - 37H9VM9WZL (Calcimycin)
RN  - 67526-95-8 (Thapsigargin)
RN  - EC 2.7.11.1 (Glycogen Synthase Kinase 3 beta)
RN  - EC 2.7.11.1 (Gsk3b protein, rat)
RN  - EC 2.7.11.26 (Glycogen Synthase Kinase 3)
RN  - EC 3.1.1.7 (Acetylcholinesterase)
RN  - G4962QA067 (Lithium Chloride)
SB  - IM
MH  - Acetylcholinesterase/genetics/*metabolism
MH  - Animals
MH  - Apoptosis/drug effects/*physiology
MH  - Calcimycin/pharmacology
MH  - Drug Interactions
MH  - Enzyme Inhibitors/pharmacology
MH  - Gene Expression/drug effects
MH  - Glycogen Synthase Kinase 3/*metabolism
MH  - Glycogen Synthase Kinase 3 beta
MH  - Iodides
MH  - Ionophores/pharmacology
MH  - Lithium Chloride/pharmacology
MH  - PC12 Cells/cytology/drug effects/enzymology
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Synapses/drug effects/*enzymology
MH  - Thapsigargin/pharmacology
MH  - Up-Regulation/drug effects
EDAT- 2007/10/24 09:00
MHDA- 2008/02/06 09:00
CRDT- 2007/10/24 09:00
PHST- 2007/10/24 09:00 [pubmed]
PHST- 2008/02/06 09:00 [medline]
PHST- 2007/10/24 09:00 [entrez]
AID - JNC4975 [pii]
AID - 10.1111/j.1471-4159.2007.04975.x [doi]
PST - ppublish
SO  - J Neurochem. 2008 Jan;104(2):409-19. doi: 10.1111/j.1471-4159.2007.04975.x. Epub 
      2007 Oct 18.