PMID- 17949490
OWN - NLM
STAT- MEDLINE
DCOM- 20080604
LR  - 20211020
IS  - 1743-422X (Electronic)
IS  - 1743-422X (Linking)
VI  - 4
DP  - 2007 Oct 19
TI  - Purification of infectious human herpesvirus 6A virions and association of host 
      cell proteins.
PG  - 101
AB  - BACKGROUND: Viruses that are incorporating host cell proteins might trigger 
      autoimmune diseases. It is therefore of interest to identify possible host 
      proteins associated with viruses, especially for enveloped viruses that have been 
      suggested to play a role in autoimmune diseases, like human herpesvirus 6A 
      (HHV-6A) in multiple sclerosis (MS). RESULTS: We have established a method for 
      rapid and morphology preserving purification of HHV-6A virions, which in 
      combination with parallel analyses with background control material released from 
      mock-infected cells facilitates qualitative and quantitative investigations of 
      the protein content of HHV-6A virions. In our iodixanol gradient purified 
      preparation, we detected high levels of viral DNA by real-time PCR and viral 
      proteins by metabolic labelling, silver staining and western blots. In contrast, 
      the background level of cellular contamination was low in the purified samples as 
      demonstrated by the silver staining and metabolic labelling analyses. Western 
      blot analyses showed that the cellular complement protein CD46, the receptor for 
      HHV-6A, is associated with the purified and infectious virions. Also, the 
      cellular proteins clathrin, ezrin and Tsg101 are associated with intact HHV-6A 
      virions. CONCLUSION: Cellular proteins are associated with HHV-6A virions. The 
      relevance of the association in disease and especially in autoimmunity will be 
      further investigated.
FAU - Hammarstedt, Maria
AU  - Hammarstedt M
AD  - Department of Biosciences and Nutrition at Novum, Karolinska Institutet, 
      Huddinge, Sweden. maria.hammarstedt@med.lu.se
FAU - Ahlqvist, Jenny
AU  - Ahlqvist J
FAU - Jacobson, Steven
AU  - Jacobson S
FAU - Garoff, Henrik
AU  - Garoff H
FAU - Fogdell-Hahn, Anna
AU  - Fogdell-Hahn A
LA  - eng
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20071019
PL  - England
TA  - Virol J
JT  - Virology journal
JID - 101231645
RN  - 0 (CD46 protein, human)
RN  - 0 (DNA, Viral)
RN  - 0 (Membrane Cofactor Protein)
SB  - IM
MH  - Blotting, Western
MH  - Cell Line
MH  - DNA, Viral/analysis/isolation & purification
MH  - Herpesvirus 6, Human/genetics/immunology/*isolation & purification
MH  - Humans
MH  - Membrane Cofactor Protein/isolation & purification/*metabolism
MH  - Multiple Sclerosis/*virology
MH  - Virion/*isolation & purification
PMC - PMC2164960
EDAT- 2007/10/24 09:00
MHDA- 2008/06/05 09:00
PMCR- 2007/10/19
CRDT- 2007/10/24 09:00
PHST- 2007/08/15 00:00 [received]
PHST- 2007/10/19 00:00 [accepted]
PHST- 2007/10/24 09:00 [pubmed]
PHST- 2008/06/05 09:00 [medline]
PHST- 2007/10/24 09:00 [entrez]
PHST- 2007/10/19 00:00 [pmc-release]
AID - 1743-422X-4-101 [pii]
AID - 10.1186/1743-422X-4-101 [doi]
PST - epublish
SO  - Virol J. 2007 Oct 19;4:101. doi: 10.1186/1743-422X-4-101.