PMID- 17951333
OWN - NLM
STAT- MEDLINE
DCOM- 20080118
LR  - 20211020
IS  - 1469-9001 (Electronic)
IS  - 1355-8382 (Print)
IS  - 1355-8382 (Linking)
VI  - 13
IP  - 12
DP  - 2007 Dec
TI  - Thiostrepton inhibition of tRNA delivery to the ribosome.
PG  - 2091-7
AB  - Ribosome-stimulated hydrolysis of guanosine-5'-triphosphate (GTP) by guanosine 
      triphosphatase (GTPase) translation factors drives protein synthesis by the 
      ribosome. Allosteric coupling of GTP hydrolysis by elongation factor Tu (EF-Tu) 
      at the ribosomal GTPase center to messenger RNA (mRNA) codon:aminoacyl-transfer 
      RNA (aa-tRNA) anticodon recognition at the ribosomal decoding site is essential 
      for accurate and rapid aa-tRNA selection. Here we use single-molecule methods to 
      investigate the mechanism of action of the antibiotic thiostrepton and show that 
      the GTPase center of the ribosome has at least two discrete functions during 
      aa-tRNA selection: binding of EF-Tu(GTP) and stimulation of GTP hydrolysis by the 
      factor. We separate these two functions of the GTPase center and assign each to 
      distinct, conserved structural regions of the ribosome. The data provide a 
      specific model for the coupling between the decoding site and the GTPase center 
      during aa-tRNA selection as well as a general mechanistic model for 
      ribosome-stimulated GTP hydrolysis by GTPase translation factors.
FAU - Gonzalez, Ruben L Jr
AU  - Gonzalez RL Jr
AD  - Department of Structural Biology, Stanford University School of Medicine, 
      Stanford, California 94305-5126, USA.
FAU - Chu, Steven
AU  - Chu S
FAU - Puglisi, Joseph D
AU  - Puglisi JD
LA  - eng
GR  - R01 GM051266/GM/NIGMS NIH HHS/United States
GR  - GM51266/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20071019
PL  - United States
TA  - RNA
JT  - RNA (New York, N.Y.)
JID - 9509184
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (RNA, Messenger)
RN  - 86-01-1 (Guanosine Triphosphate)
RN  - 9014-25-9 (RNA, Transfer)
RN  - EC 3.6.1.- (GTP Phosphohydrolases)
RN  - EC 3.6.1.- (Peptide Elongation Factor Tu)
RN  - HR4S203Y18 (Thiostrepton)
SB  - IM
MH  - Anti-Bacterial Agents/pharmacology
MH  - GTP Phosphohydrolases/metabolism
MH  - Guanosine Triphosphate/metabolism
MH  - Models, Molecular
MH  - Molecular Biology
MH  - Nucleic Acid Conformation
MH  - Peptide Elongation Factor Tu/drug effects/metabolism
MH  - RNA, Messenger/*genetics
MH  - RNA, Transfer/drug effects/*genetics
MH  - Ribosomes/drug effects/*physiology
MH  - Spectrometry, Fluorescence
MH  - Thiostrepton/*pharmacology
PMC - PMC2080598
EDAT- 2007/10/24 09:00
MHDA- 2008/01/19 09:00
PMCR- 2008/06/01
CRDT- 2007/10/24 09:00
PHST- 2007/10/24 09:00 [pubmed]
PHST- 2008/01/19 09:00 [medline]
PHST- 2007/10/24 09:00 [entrez]
PHST- 2008/06/01 00:00 [pmc-release]
AID - rna.499407 [pii]
AID - RA [pii]
AID - 10.1261/rna.499407 [doi]
PST - ppublish
SO  - RNA. 2007 Dec;13(12):2091-7. doi: 10.1261/rna.499407. Epub 2007 Oct 19.