PMID- 17951581
OWN - NLM
STAT- MEDLINE
DCOM- 20080310
LR  - 20220316
IS  - 0268-1161 (Print)
IS  - 0268-1161 (Linking)
VI  - 22
IP  - 12
DP  - 2007 Dec
TI  - Gene expression in human cumulus cells: one approach to oocyte competence.
PG  - 3069-77
AB  - BACKGROUND: Dialogue between the oocyte and cumulus cells is essential for oocyte 
      maturation. A prospective laboratory research project was designed to evaluate 
      transcription of specific genes in cumulus cells harvested before 
      intracytoplasmic sperm injection from pre-ovulatory follicles, according to 
      individual oocyte nuclear maturity and developmental competence. Genes were 
      chosen because their expression was induced by the LH peak [Steroidogenic Acute 
      Regulatory protein (STAR), Cyclooxygenase 2 (COX2 or PTGS2), Amphiregulin (AREG)] 
      or because they were involved in oocyte lipidic metabolism [Stearoyl-Coenzyme A 
      Desaturase 1 and 5 (SCD1 and SCD5)] or in gap-junctions [Connexin 43 (CX43 or 
      GJA1)]. METHODS: mRNA levels in cumulus cells were assessed by real-time PCR. 
      RESULTS: Expression levels of all genes investigated, except Cx43, were increased 
      after resumption of meiosis. Nuclear maturation was thus associated with 
      increased expression of STAR, COX2, AREG, SCD1 and SCD5 by cumulus cells. When 
      considering only cumulus associated with metaphase II oocytes, gene expression 
      was independent of morphological status at Day 2. In contrast, transcript levels 
      were lower and distributed over a narrower range in cumulus enclosing oocytes 
      achieving blastocyst development at Day 5/6 than in cumulus enclosing oocytes 
      unable to develop beyond the embryo stage. CONCLUSION: Further developmental 
      potential from embryo to blastocyst stage was associated with lower expression in 
      a narrow range for these genes.
FAU - Feuerstein, P
AU  - Feuerstein P
AD  - INRA, CNRS, Universite de Tours, Haras Nationaux, UMR Physiologie de la 
      Reproduction et des Comportements, 37380 Nouzilly, France.
FAU - Cadoret, V
AU  - Cadoret V
FAU - Dalbies-Tran, R
AU  - Dalbies-Tran R
FAU - Guerif, F
AU  - Guerif F
FAU - Bidault, R
AU  - Bidault R
FAU - Royere, D
AU  - Royere D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20071019
PL  - England
TA  - Hum Reprod
JT  - Human reproduction (Oxford, England)
JID - 8701199
RN  - 0 (AREG protein, human)
RN  - 0 (Amphiregulin)
RN  - 0 (Connexin 43)
RN  - 0 (EGF Family of Proteins)
RN  - 0 (GJA1 protein, human)
RN  - 0 (Glycoproteins)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (Phosphoproteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (steroidogenic acute regulatory protein)
RN  - EC 1.14.19.1 (SCD1 protein, human)
RN  - EC 1.14.19.1 (SCD5 protein, human)
RN  - EC 1.14.19.1 (Stearoyl-CoA Desaturase)
RN  - EC 1.14.99.1 (Cyclooxygenase 2)
SB  - IM
MH  - Adult
MH  - Amphiregulin
MH  - Cell Communication/*physiology
MH  - Connexin 43/genetics
MH  - Cumulus Cells/*cytology/*physiology
MH  - Cyclooxygenase 2/genetics
MH  - EGF Family of Proteins
MH  - Female
MH  - Gene Expression Profiling
MH  - Gene Expression Regulation, Developmental/*physiology
MH  - Glycoproteins/genetics
MH  - Humans
MH  - Intercellular Signaling Peptides and Proteins/genetics
MH  - Meiosis/physiology
MH  - Oocytes/*cytology
MH  - Phosphoproteins/genetics
MH  - RNA, Messenger/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sperm Injections, Intracytoplasmic
MH  - Stearoyl-CoA Desaturase/genetics
MH  - Transcription, Genetic
EDAT- 2007/10/24 09:00
MHDA- 2008/03/11 09:00
CRDT- 2007/10/24 09:00
PHST- 2007/10/24 09:00 [pubmed]
PHST- 2008/03/11 09:00 [medline]
PHST- 2007/10/24 09:00 [entrez]
AID - dem336 [pii]
AID - 10.1093/humrep/dem336 [doi]
PST - ppublish
SO  - Hum Reprod. 2007 Dec;22(12):3069-77. doi: 10.1093/humrep/dem336. Epub 2007 Oct 
      19.