PMID- 17951872
OWN - NLM
STAT- MEDLINE
DCOM- 20080122
LR  - 20131121
IS  - 0273-1223 (Print)
IS  - 0273-1223 (Linking)
VI  - 56
IP  - 7
DP  - 2007
TI  - Free ammonia and free nitrous acid inhibition on the anabolic and catabolic 
      processes of Nitrosomonas and Nitrobacter.
PG  - 89-97
AB  - The inhibitory effects of free ammonia (FA) and free nitrous acid (FNA) on the 
      catabolic and anabolic processes of Nitrosomonas and Nitrobacter were 
      investigated using a method that allows decoupling the growth and energy 
      generation processes. Lab-scale sequencing batch reactors (SBRs) were operated 
      for the enrichment of Nitrosomonas and Nitrobacter. Fluorescent In-Situ 
      Hybridization (FISH) analysis showed that the reactors were 82% and 73% enriched 
      with Nitrosomonas and Nitrobacter, respectively. Batch tests were carried out to 
      measure the oxygen uptake rate (OUR) by the enriched cultures at various FA and 
      FNA levels, in the presence (OUR with CO2 ) or absence (OUR without CO2) of 
      inorganic carbon (CO2, HCO*3 and CO 2*3). FA up to 16.0 mgNH3-N.L(-1) was not 
      found to have any inhibitory effect on either the catabolic or anabolic processes 
      of the Nitrosomonas culture, but both these processes were inhibited by FNA. 
      While an FNA level of 0.40-0.63 mgHNO2-N.L(-1) inhibited the energy production 
      capability of Nitrosomonas by 50%, the growth process of the culture was 
      completely inhibited by FNA at a concentration of 0.40 mgHNO2-N.L(-1). Both FA 
      and FNA were found to have strong inhibition on the anabolic processes of 
      Nitrobacter, but with limited inhibitory effects on the catabolism of this 
      culture. The biosynthesis of Nitrobacter was totally inhibited at an FA level of 
      6.0 mgNH3-N.L(-1) (or above) or an FNA level of 0.02 mgHNO2-N.L(-1) (or above). 
      At the same level of FA, the energy production capability of Nitrobacter was only 
      inhibited by 12%, whereas an FNA level of up to 0.024 mgHNO2-N.L(-1) did not show 
      any inhibition on the energy production of Nitrobacter. Further, these inhibitory 
      effects appears to be much stronger on Nitrobacter than on Nitrosomonas, 
      supporting that FA and FNA inhibition may play a major role in the elimination of 
      nitrite oxidizing bacteria in processes treating wastewater containing a high 
      level of nitrogen.
FAU - Vadivelu, V M
AU  - Vadivelu VM
AD  - The Advanced Wastewater Management Centre, The University of Queensland, St 
      Lucia, QLD 4072, Australia.
FAU - Keller, J
AU  - Keller J
FAU - Yuan, Z
AU  - Yuan Z
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Water Sci Technol
JT  - Water science and technology : a journal of the International Association on 
      Water Pollution Research
JID - 9879497
RN  - 0 (Anabolic Agents)
RN  - 142M471B3J (Carbon Dioxide)
RN  - 7664-41-7 (Ammonia)
RN  - S88TT14065 (Oxygen)
RN  - T2I5UM75DN (Nitrous Acid)
SB  - IM
MH  - Ammonia/*pharmacology
MH  - Anabolic Agents/*pharmacology
MH  - Carbon Dioxide/pharmacology
MH  - Nitrobacter/*drug effects/*metabolism
MH  - Nitrosomonas/*drug effects/*metabolism
MH  - Nitrous Acid/*pharmacology
MH  - Oxygen/metabolism
EDAT- 2007/10/24 09:00
MHDA- 2008/01/23 09:00
CRDT- 2007/10/24 09:00
PHST- 2007/10/24 09:00 [pubmed]
PHST- 2008/01/23 09:00 [medline]
PHST- 2007/10/24 09:00 [entrez]
AID - 10.2166/wst.2007.612 [doi]
PST - ppublish
SO  - Water Sci Technol. 2007;56(7):89-97. doi: 10.2166/wst.2007.612.